Morphology and molecular phylogeny of Nitzschia bizertensis sp. nov.—A new domoic acid-producer

Autor: Asma Sakka Hlaili, Christophe Lambert, Nina Lundholm, Yuichi Kotaki, Donia Bouchouicha Smida, Hassine Hadj Mabrouk, Inès Sahraoui, Marianne Ellegaard, Maria Valeria Ruggiero, Wiebe H. C. F. Kooistra
Přispěvatelé: Faculté des Sciences de Bizerte [Université de Carthage], Université de Carthage - University of Carthage, Natural History Museum of Denmark, Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH), Stazione Zoologica Anton Dohrn (SZN), Kitasato University, Department of Plant and Environmental Sciences [Copenhagen], Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), European Project: 201724,EC:FP7:ENV,FP7-ENV-2007-1,MIDTAL(2008), University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2014
Předmět:
Zdroj: Harmful Algae
Harmful Algae, 2014, 32, pp.49-63. ⟨10.1016/j.hal.2013.12.004⟩
Harmful Algae, Elsevier, 2014, 32, pp.49-63. ⟨10.1016/j.hal.2013.12.004⟩
ISSN: 1568-9883
Popis: International audience; A new toxin-producing marine diatom, Nitzschia bizertensis sp. nov., isolated from the Bizerte Lagoon (Tunisia, Southwest Mediterranean Sea) is, based on studies on eight different strains, characterized morphologically by light microscopy, transmission and scanning electron microscopy, and phylogeneti- cally using the nuclear rDNA regions: SSU, ITS1, 5.8S, ITS2 and D1–D3 of the LSU. The species belongs to the sections Lanceolatae or Lineares as defined by Cleve and Grunow (1880). These sections are characterized by species having linear-lanceolate valves with an eccentric raphe where the fibulae does not extend into the valve, and are otherwise famous for the lack of characters useful for delineation of species. Nitzschia bizertensis differs from most other species in these sections by having a high density of interstriae. The morphological and phylogenetic studies and comparisons with previously described Nitzschia species showed Nitzschia bizertensis sp. nov. to be a new species. Batch culture experiments were conducted for estimations of maximum growth rate and production of domoic acid (DA). Maximum cellular DA content of the examined strains ranged from 2 x 10-4 to 3.6 x 10-2 pg cells-1. The total DA concentration (pg mL-1) was high already in exponential growth phase maybe due to reinoculation of ‘‘old’’ stationary phase cells, and increased into stationary growth phase where it reached a stationary level varying among the strains from ca. 4500 to 9500 pg mL-1. Nitzschia bizertensis represents a new domoic acid-producing diatom and is the second toxin producing Nitzschia species. The resolution of Nitzschia bizertensis and Nitzschia navis-varingica in different parts of the LSU phylogenetic tree, and the recovery of the Pseudo-nitzschia species phylogenetically distant from those two species suggests that the ability to produce DA either evolved multiple times independently or was lost multiple times.
Databáze: OpenAIRE
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