Large-scale physical mapping within the region 22q12.3–13.1 in meningioma
| Autor: | Nikolaus Blin, Elisabeth Göttert, Jeffrey M. Trent, Ruth Herzog, Klaus D. Zang, E. Meese, Wolfram Henn |
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| Rok vydání: | 1991 |
| Předmět: |
Genetic Markers
Genetic Linkage Chromosomes Human Pair 22 Restriction Mapping Hybrid Cells Biology Mice Gene mapping Meningeal Neoplasms Genetics Pulsed-field gel electrophoresis Animals Humans Gene Cells Cultured Skin Electrophoresis Agar Gel Platelet-Derived Growth Factor Gel electrophoresis PDGFB PDGFB Gene Nucleic Acid Hybridization DNA Neoplasm Chromosome Banding Blotting Southern Chromosomal region Meningioma Chromosome 22 |
| Zdroj: | Genomics. 10:1041-1046 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/0888-7543(91)90196-l |
| Popis: | The lack of physical mapping data strongly restricts the analysis of the meningioma chromosomal region that was assigned to the bands 22q12.3-qter. Recently, we reported a new marker D22S16 for chromosome 22 that was assigned to the region 22q13-qter by in situ hybridization. Utilizing somatic cell hybrids we now sublocalized the marker D22S16 within the band region 22q12–13.1, thus placing it in the vicinity of the gene for the platelet derived growth factor (PDGFB). A physical map was established for the regions surrounding the PDGFB gene and the D22S16 marker. By means of pulsed-field gel electrophoresis (PFGE) D22S16 and PDGFB were found to be physically linked within 900 kb. We also identified two CpG clusters bordering the PDGFB gene. For the enzyme Not I, a variation of the PDGFB restriction pattern was found between different individuals. PFGE analysis of the two loci (PDFGB and D22S16) failed to identify major rearrangements in meningioma. |
| Databáze: | OpenAIRE |
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