Fibrin hydrogels are safe, degradable scaffolds for sub-retinal implantation

Autor: Jarel K. Gandhi, Alan D. Marmorstein, Timothy W. Olsen, Michael P. Fautsch, Raymond Iezzi, Jose S. Pulido, Fukutaro Mano, Brad H. Holman, Stephen A. LoBue
Rok vydání: 2019
Předmět:
Scaffold
Medical Implants
Swine
Sus scrofa
Biochemistry
chemistry.chemical_compound
Macular Degeneration
0302 clinical medicine
Absorbable Implants
Medicine and Health Sciences
Geriatric Ophthalmology
Materials
Mammals
0303 health sciences
Multidisciplinary
biology
Tissue Scaffolds
Chemistry
Retinal Degeneration
Eukaryota
Equipment Design
medicine.anatomical_structure
Self-healing hydrogels
Physical Sciences
Vertebrates
Medicine
Engineering and Technology
Retinal Disorders
Female
Anatomy
Research Article
Biotechnology
Science
Ocular Anatomy
Amorphous Solids
Materials Science
Bioengineering
Fibrin
Retina
03 medical and health sciences
Ocular System
medicine
Animals
030304 developmental biology
Retinal pigment epithelium
Organisms
Biology and Life Sciences
Proteins
Retinal
eye diseases
Transplantation
Ophthalmology
Geriatrics
Mixtures
Macular Disorders
Amniotes
030221 ophthalmology & optometry
biology.protein
Eyes
Medical Devices and Equipment
Implant
sense organs
Head
Gels
Biomedical engineering
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 1, p e0227641 (2020)
ISSN: 1932-6203
Popis: Retinal pigment epithelium (RPE) transplantation for the treatment of macular degeneration has been studied for over 30 years. Human clinical trials have demonstrated that RPE monolayers exhibit improved cellular engraftment and survival compared to single cell suspensions. The use of a scaffold facilitates implantation of a flat, wrinkle-free, precisely placed monolayer. Scaffolds currently being investigated in human clinical trials are non-degradable which results in the introduction of a chronic foreign body. To improve RPE transplant technology, a degradable scaffold would be desirable. Using human fibrin, we have generated scaffolds that support the growth of an RPE monolayer in vitro. To determine whether these scaffolds are degraded in vivo, we developed a surgical approach that delivers a fibrin hydrogel implant to the sub-retinal space of the pig eye and determined whether and how fast they degraded. Using standard ophthalmic imaging techniques, the fibrin scaffolds were completely degraded by postoperative week 8 in 5 of 6 animals. Postmortem histologic analysis confirmed the absence of the scaffold from the subretinal space at 8 weeks, and demonstrated the reattachment of the neurosensory retina and a normal RPE-photoreceptor interface. When mechanical debridement of a region of native RPE was performed during implantation surgery degradation was accelerated and scaffolds were undetectable by 4 weeks. These data represent the first in situ demonstration of a fully biodegradable scaffold for use in the implantation of RPE and other cell types for treatment of macular degeneration and other retinal degenerative diseases.
Databáze: OpenAIRE
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