Cloning of a Third Mammalian Na+-Ca2+ Exchanger, NCX3
| Autor: | Kenneth D. Philipson, Beate D. Quednau, Aldons J. Lusis, Yu-Rong Xia, Debora A. Nicoll, Zhiyong Qui |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Gene isoform
DNA Complementary Molecular Sequence Data Clone (cell biology) Biology Biochemistry Sodium Channels Sodium-Calcium Exchanger Cell Line Cricetinae Complementary DNA Animals Amino Acid Sequence Cloning Molecular Molecular Biology Gene Cloning chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid cDNA library Sodium Chromosome Mapping Membrane Transport Proteins Cell Biology Molecular biology Rats Amino acid Open reading frame chemistry Calcium Calcium Channels |
| Zdroj: | Journal of Biological Chemistry. 271:24914-24921 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.271.40.24914 |
| Popis: | NCX3 is the third isoform of a mammalian Na+-Ca2+ exchanger to be cloned. NCX3 was identified from rat brain cDNA by polymerase chain reaction (PCR) using degenerate primers derived from the sequences of two conserved regions of NCX1 and NCX2. The NCX3 PCR product was used to isolate two overlapping clones totalling 4.8 kilobases (kb) from a rat brain cDNA library. The overlapping clones were sequenced and joined at a unique Bsp106I restriction enzyme site to form a full-length cDNA clone. The NCX3 cDNA clone has an open reading frame of 2.8 kb encoding a protein of 927 amino acids. At the amino acid level, NCX3 shares 73% identity with NCX1 and 75% identity with NCX2 and is predicted to share the same membrane topology as NCX1 and NCX2. Following addition of a poly(A)+ tail to the NCX3 clone, exchanger activity could be expressed in Xenopus oocytes. NCX3 was also expressed in the mammalian BHK cell line. NCX3 transcripts are 6 kb in size and are highly restricted to brain and skeletal muscle. Linkage analysis in the mouse indicated that the NCX family of genes is dispersed, since the NCX1, NCX2, and NCX3 genes mapped to mouse chromosomes 17, 7, and 12, respectively. |
| Databáze: | OpenAIRE |
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