Characterization of osteoblastic properties of 7F2 and UMR-106 cultures after acclimation to reduced levels of fetal bovine serum
| Autor: | L A Ashley, David K. Peyton, D L DeMoss, R D Grey, C M Pendleton, Sourik S. Ganguly, G C Howard, L D Castle, M.E. Fultz |
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| Rok vydání: | 2008 |
| Předmět: |
Genetic Markers
Serum medicine.medical_specialty Cell Survival Physiology medicine.drug_class Osteocalcin Biology Ovarian hormone Acclimatization Cell Line Mice Pregnancy Physiology (medical) Internal medicine medicine Animals Pharmacology Fetus Osteoblasts Estradiol Reverse Transcriptase Polymerase Chain Reaction RANK Ligand Cell Differentiation Osteoblast General Medicine Alkaline Phosphatase Fetal Blood Adaptation Physiological Immunohistochemistry medicine.anatomical_structure Endocrinology Cell culture Estrogen Cattle Female Fetal bovine serum |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 86:403-415 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/y08-055 |
| Popis: | Estrogen plays an important role in skeletal physiology by maintaining a remodeling balance between the activity of osteoblasts and osteoclasts. In an attempt to decipher the mechanism through which estrogen elicits its action on osteoblasts, experimentation necessitated the development of a culturing environment reduced in estrogenic compounds. The selected medium (OPTI-MEM) is enriched to sustain cultures under reduced fetal bovine serum (FBS) conditions and is devoid of the pH indicator phenol red, a suspected estrogenic agent. This protocol reduced the concentration of FBS supplementation to 0% through successive 24 h incubations with diminishing amounts of total FBS (1%, 0.1%, and 0%). The protocol does not appear to alter the viability, cell morphology, or osteoblast-like phenotype of 7F2 and UMR-106 cell lines when compared with control cells grown in various concentrations of FBS. Although the rate of mitotic divisions declined, the 7F2 and UMR-106 cultures continued to express osteoblast-specific markers and exhibited estrogen responsiveness. These experimental findings demonstrate that the culture protocol developed did not alter the osteoblast nature of the cell lines and provides a model system to study estrogen’s antiresorptive role on skeletal turnover. |
| Databáze: | OpenAIRE |
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