Inhibitory effects of isoliquiritigenin on the migration and invasion of human breast cancer cells

Autor: Chia Jung Chan, Kai-Lee Wang, Chih Yang Huang, Full Young Chang, Da Tian Bau, Paulus S. Wang, Shih Min Hsia
Rok vydání: 2013
Předmět:
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Clinical Biochemistry
Breast Neoplasms
Matrix Metalloproteinase Inhibitors
Matrix metalloproteinase
Cell Line
Metastasis
chemistry.chemical_compound
Chalcones
Breast cancer
Western blot
Cell Movement
Cell Line
Tumor
Internal medicine
Drug Discovery
medicine
Humans
Neoplasm Invasiveness
Phosphorylation
Mammary Glands
Human
Pharmacology
Wound Healing
medicine.diagnostic_test
business.industry
Hypoxia-Inducible Factor 1
alpha Subunit
medicine.disease
Antineoplastic Agents
Phytogenic
Neoplasm Proteins
Vascular endothelial growth factor
Endocrinology
Matrix Metalloproteinase 9
chemistry
Cell culture
Cancer cell
Cancer research
Matrix Metalloproteinase 2
Molecular Medicine
Female
business
Protein Processing
Post-Translational
Isoliquiritigenin
Signal Transduction
Zdroj: Expert Opinion on Therapeutic Targets. 17:337-349
ISSN: 1744-7631
1472-8222
DOI: 10.1517/14728222.2013.756869
Popis: Isoliquiritigenin (ISL) is a natural phenolic compound extracted from licorice. Previous studies have shown that ISL is a potent antioxidant with anti-inflammatory and antitumor activities. The anti-invasive activity of ISL was still unclear. The actual causes of death for most breast cancer patients were due to the tumor metastasis. Attenuating the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs) is well known to prevent tumor metastasis.The purpose of this study is to investigate the effects of ISL on VEGF and MMP expression in highly metastatic human breast cancer cell line, MDA-MB-231.ISL reduced the secretions and protein levels of VEGF. The VEGF upstream regulatory protein, hypoxia-inducible factor 1-alpha (HIF-1α), was also reduced after ISL treatment. Moreover, ISL inhibited the expression and gelatinolytic activity of MMP-2 and MMP-9 which were confirmed by western blot and gelatin zymography assay. Additionally, the anti-migratory activity of ISL was further confirmed by chamber migration assay and wound migration assay. Upstream signaling pathways, including the expression of phosphatidylinositol-3 kinase (PI3K), the phosphorylation of p38 and Akt kinase and NF-κB DNA binding activity, were suppressed by ISL.These findings suggest that ISL suppresses the migration of MDA-MB-231 cells by inhibiting the upstream signaling pathways.
Databáze: OpenAIRE
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