Sensing of pathogenic bacteria based on their interaction with supported bilayer membranes studied by impedance spectroscopy and surface plasmon resonance
| Autor: | Thet Naing Tun, Petra J. Cameron, A. Tobias A. Jenkins |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Staphylococcus aureus
Bacterial Toxins Lipid Bilayers Biomedical Engineering Biophysics 02 engineering and technology Biology medicine.disease_cause Hemolysis Cell membrane Hemolysin Proteins 03 medical and health sciences Electrochemistry medicine Humans Secretion Surface plasmon resonance Lipid bilayer 030304 developmental biology 0303 health sciences Pore-forming toxin Bilayer Pathogenic bacteria General Medicine Surface Plasmon Resonance 021001 nanoscience & nanotechnology Phospholipases A2 Membrane medicine.anatomical_structure Biochemistry Dielectric Spectroscopy Pseudomonas aeruginosa 0210 nano-technology Biotechnology |
| Zdroj: | Tun, T N, Cameron, P J & Jenkins, A T A 2011, ' Sensing of pathogenic bacteria based on their interaction with supported bilayer membranes studied by impedance spectroscopy and surface plasmon resonance ', Biosensors and Bioelectronics, vol. 28, no. 1, pp. 227-231 . https://doi.org/10.1016/j.bios.2011.07.023 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2011.07.023 |
| Popis: | Pathogenic bacteria secrete various virulence factors, including toxins, lipases and proteases that allow them to infect, breakdown and colonize host tissue. Among various modes of action that the pathogenic bacteria use to damage the host, pore formation (by pore forming toxins (PFTs)) and lipid hydrolysis (by phospholipases) modes are common in damaging the eukaryotic cell membrane. PFTs in their monomeric form are extracellular diffusible and able to form hydrophilic pores in cell membrane while phospholipases cleaves and hydrolyzes the ester bonds of most phospholipids in cell membrane. Both modes of action cause uncontrolled permeation of ions and molecules across cell membrane, leading to cell death by apoptosis or necrosis. In this work, the toxins secreted by two clinically important human pathogens, methicillin susceptible Staphylococcus aureus (MSSA476) and Pseudomonas aeruginosa (PAO1) were studied via their interaction with a planar tethered bilayer lipid membrane (pTBLM) using surface plasmon resonance spectroscopy (SPR) and electrochemical impedance spectroscopy (EIS). Detection and discrimination is based on lipid-loss (lipid hydrolysis by phospholipases) or non lipid-loss (pore formation by PFTs) from pTBLM upon interaction with supernatant of pathogenic bacteria. Using EIS and SPR, it is demonstrated that major toxins of S. auerus are PFTs while most of toxin associated with P. aeruginosa are more lipid damaging lipolytic enzymes. Such a format might have future utility as a simple assay for measuring the presence membrane lytic virulence factors in clinical samples. |
| Databáze: | OpenAIRE |
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