Fatty acid-binding protein regulates LPS-induced TNF-α production in mast cells
| Autor: | Hisatake Kondo, Masao Ono, Keiju Motohashi, Hiroyuki Sakagami, Yuji Owada, Tomoo Sawada, Yoshiya Ueyama, Izumi Kaneko, Hiroshi Furukawa, Yasuhiro Adachi, Nobuko Tokuda, Kohji Fukunaga, Noriko Yamamoto |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Male Time Factors Lipopolysaccharide medicine.medical_treatment Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Stimulation Peritonitis Biology Fatty Acid-Binding Proteins Fatty acid-binding protein Mice chemistry.chemical_compound Immune system In vivo Sepsis medicine Animals Mast Cells Cells Cultured Mice Knockout Dose-Response Relationship Drug Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Cell Biology Mast cell Immunohistochemistry Cell biology Mice Inbred C57BL Survival Rate medicine.anatomical_structure Cytokine chemistry Immunology lipids (amino acids peptides and proteins) Signal transduction |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 79:21-26 |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/j.plefa.2008.06.003 |
| Popis: | There has been increasing evidence for the involvement of fatty acid-binding proteins (FABPs) in the cytokine production of macrophages and dendritic cells probably through the control of cellular lipid metabolism and signal transduction. Since mast cells (MCs) are recently shown to be involved in immune response through modification of cytokine production, it is possible that some FABPs could also be involved in the immune response of MCs. In this study, we found that epidermal-type FABP (E-FABP) was expressed in murine bone marrow-derived MCs (BMMCs). Using BMMCs from genetically E-FABP-null mutated mice, we demonstrated that E-FABP in BMMCs plays a key role in the production of TNF-alpha following lipopolysaccharide (LPS) stimulation. In the in vivo septic peritonitis model (cecal ligation and puncture model), E-FABP-null mice showed a significantly increased mortality compared to wild-type mice. However, no significant difference in antigen-induced cytokine production was observed between wild-type and E-FABP-null BMMCs, and systemic anaphylaxis was equally induced in vivo in both wild-type and E-FABP-null mice. These results suggest that E-FABP is specifically involved in the LPS-induced cytokine production of MCs, and could play a role in the host-defense against bacterial infection, possibly through regulation of TNF-alpha production. |
| Databáze: | OpenAIRE |
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