Intranasal Anti-rabies DNA Immunization Promotes a Th1-related Cytokine Stimulation Associated with Plasmid Survival Time
| Autor: | Iris A. Feria-Romero, Fernando Calzada-Bermejo, Sandra Orozco-Suárez, Karina Chávez-Rueda, Francisco Blanco-Favela, Alvaro Aguilar-Setién, Luis Chávez-Sánchez, Rafael Hernández-González, Leticia Manuel-Apolinar, Emiliano Tesoro-Cruz |
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| Rok vydání: | 2011 |
| Předmět: |
Immunocytochemistry
Spleen Biology Polymerase Chain Reaction law.invention DNA vaccination Mice Plasmid law Vaccines DNA medicine Animals Neutralizing antibody Administration Intranasal Polymerase chain reaction Mice Inbred BALB C General Medicine Th1 Cells medicine.disease Antibodies Neutralizing Immunohistochemistry Virology medicine.anatomical_structure Rabies Vaccines Immunology biology.protein Cytokines Rabies Antibody |
| Zdroj: | Archives of Medical Research. 42:563-571 |
| ISSN: | 0188-4409 |
| DOI: | 10.1016/j.arcmed.2011.10.009 |
| Popis: | Background and Aims DNA vaccination has a great potential to decrease infectious diseases worldwide, such as rabies. Here we showed the effects of a single anti-rabies DNA vaccination applied intranasally (IN) on plasmid survival time, neutralizing antibody (NA) titers, G-protein expression and Th1/Th2-related cytokines. Methods Only one 50-μg dose of an anti-rabies DNA vaccine was IN administered to 160 Balb/c mice. Twenty mice were used for the neutralizing antibody study, 35 for the proliferation assay, 35 for Th1/Th2-related cytokines, 35 for glycoprotein expression by immunocytochemistry, and 35 for pGQH detection and G-protein mRNA expression. Results Th1-type related cytokines from spleen cells (IFN-γ, TNF-α, and IL-2) were detected. Rabies NA titers were ≥0.6 IUs from day 30 onward in the IN DNA-vaccinated group. The plasmid was identified in brains and lungs from days 3–15. The mRNA transcript was amplified in brains and lungs from days 3–30, and G-protein expression was observed in spleens, brains and lungs on days 3, 8, and 15. In all cases, a gradual decrease was observed on days 30 and 45 and absent on day 60. Conclusions We found that Th1-type related cytokines (IL-2, IFN-γ, and TNF-α) were stimulated during the first month after DNA vaccination, correlating with the proliferation assays. Also, it was associated with the plasmid survival time remaining in lungs and brains prior to its degradation. |
| Databáze: | OpenAIRE |
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