Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland
| Autor: | James I. Nagy, Deborah D. Tsao, S. G. Wang, B.D. Lynn |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Calbindins Pathology medicine.medical_specialty Connexin Pineal Gland Calbindin Pinealocyte Rats Sprague-Dawley Mice 03 medical and health sciences Pineal gland symbols.namesake 0302 clinical medicine medicine Animals Perivascular space Cellular localization Glial fibrillary acidic protein biology General Neuroscience Gap Junctions Interstitial Cells of Cajal Rats Interstitial cell of Cajal Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure nervous system Connexin 43 cardiovascular system biology.protein symbols sense organs 030217 neurology & neurosurgery |
| Zdroj: | European Journal of Neuroscience. 45:1553-1569 |
| ISSN: | 0953-816X |
| Popis: | Gap junctions between cells in the pineal gland have been described ultrastructurally, but their connexin constituents have not been fully characterized. We used immunofluorescence in combination with markers of pineal cells to document the cellular localization of connexin43 (Cx43). Immunofluorescence labelling of Cx43 with several different antibodies was widely distributed throughout the pineal, whereas another connexin examined, connexin26, was not found in pineal but only in surrounding leptomeninges. Labelling apparently associated with plasma membranes was visualized either as fine Cx43-puncta (1-2 μm) or as unusually large pools of Cx43 ranging up to 4-7 μm in diameter or length. These puncta and pools were highly concentrated in perivascular spaces, where they were associated with numerous cells devoid of labelling for markers of pinealocytes (e.g. tryptophan hydroxylase and serotonin), and where they were minimally associated with blood vessels and lacked association with resident macrophages. Astrocytes labelled for glial fibrillary acidic protein were largely restricted to the anterior pole of the pineal gland, where they displayed only fine and sparse Cx43-puncta along their processes. Labelling for Cx43 was localized largely though not exclusively to the somata and long processes of a subpopulation of perivascular interstitial cells that were immunopositive for calbindin-D28K. These cells were often located among dense bundles or termination areas of sympathetic fibres labelled for tyrosine hydroxylase or serotonin. The results indicate that interstitial cells form abundant gap junctions composed of Cx43, and suggest that gap junction-mediated intracellular communication by these cells supports the activities of pinealocytes. |
| Databáze: | OpenAIRE |
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