Global analysis of the Deinococcus radiodurans proteome by using accurate mass tags
| Autor: | Harold R. Udseth, John R. Battista, Mary S. Lipton, Kwong Kwok Wong, Richard D. Smith, Amudhan Venkateswaran, Lye Meng Markillie, Deanna L. Auberry, Michael J. Daly, Rui Zhao, Heather M. Kostandarithes, Ronald J. Moore, James K. Fredrickson, Ljiljana Paša-Tolić, Eric Stritmatter, Yufeng Shen, Kim K. Hixson, Gordon A. Anderson, Christophe Masselon, David J. Anderson, Nikola Tolić, Margaret F. Romine |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Proteome Computational biology Mass spectrometry Radiation Tolerance Fourier transform ion cyclotron resonance Bacterial protein Bacterial Proteins Trypsin Organism Genetics Multidisciplinary Bacteria Enzymatic digestion biology RNA Ribosomal 5S Chromosome Mapping Deinococcus radiodurans DNA-Directed RNA Polymerases Biological Sciences biology.organism_classification RNA Bacterial Kinetics Rec A Recombinases Commentary Capillary Action Ion cyclotron resonance |
| Zdroj: | Proceedings of the National Academy of Sciences. 99:11049-11054 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.172170199 |
| Popis: | Understanding biological systems and the roles of their constituents is facilitated by the ability to make quantitative, sensitive, and comprehensive measurements of how their proteome changes, e.g., in response to environmental perturbations. To this end, we have developed a high-throughput methodology to characterize an organism's dynamic proteome based on the combination of global enzymatic digestion, high-resolution liquid chromatographic separations, and analysis by Fourier transform ion cyclotron resonance mass spectrometry. The peptides produced serve as accurate mass tags for the proteins and have been used to identify with high confidence >61% of the predicted proteome for the ionizing radiation-resistant bacterium Deinococcus radiodurans . This fraction represents the broadest proteome coverage for any organism to date and includes 715 proteins previously annotated as either hypothetical or conserved hypothetical. |
| Databáze: | OpenAIRE |
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