A yeast gene (BEM1) necessary for cell polarization whose product contains two SH3 domains
| Autor: | Janet Chenevert, Kathleen Corrado, John R. Pringle, Ira Herskowitz, Alan Bender |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Multidisciplinary Base Sequence biology Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae Mutant Cell Polarity Cell cycle biology.organism_classification Molecular biology Cell biology Fungal Proteins Mutation Cell polarity Extracellular Amino Acid Sequence Cytoskeleton Sequence Alignment Actin Intracellular Adaptor Proteins Signal Transducing |
| Zdroj: | Nature. 356:77-79 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/356077a0 |
| Popis: | CELL polarization requires that a cellular axis or cell-surface site be chosen and that the cytoskeleton be organized with respect to it. Details of the link between the cytoskeleton and the chosen axis or site are not clear1. Cells of the yeast Saccharomyces cerevisiae exhibit cell polarization in two phases of their life cycle, during vegetative growth and during mating, which reflects responses to intracellular and extracellular signals, respectively. Here we describe the isolation of two mutants defective specifically in cell polarization in response to peptide mating pheromones. The mutants carry special alleles (denoted bem1-s) of the BEM1 gene required for cell polarization during vegetative growth2,3. Unlike other beml mutants, the bem1-s mutants are normal for vegetative growth. Complete deletion of BEM1 leads to the defect in polarization of vegetative cells seen in beml mutants23. The predicted sequence of the BEM1 protein (Bem1p) reveals two copies of a domain (denoted SH3) that is found in many proteins associated with the cortical cytoskeleton and which may mediate binding to actin or some other component of the cell cortex4,5. The sequence of Bemlp and the properties of mutants defective in this protein indicate that it may link the cytoskeleton to morphogenetic determinants on the cell surface. |
| Databáze: | OpenAIRE |
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