Differential binding of human blood group Sd(a+) and Sd(a−) Tamm-Horsfall glycoproteins withDolichos biflorusandVicia villosa-B4agglutinins
| ISSN: | 0014-5793 |
|---|---|
| DOI: | 10.1016/s0014-5793(98)00617-6 |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d8df21941f49f392ce0dedc9b6d614c https://doi.org/10.1016/s0014-5793(98)00617-6 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....2d8df21941f49f392ce0dedc9b6d614c |
| Autor: | Chie-Pein Chen, Albert M. Wu, Shuh-Chyung Song, June H. Wu, Yuen-Yuen Chen, Winifred M. Watkins |
| Rok vydání: | 1998 |
| Předmět: |
Acetylgalactosamine
Tamm–Horsfall protein Molecular Sequence Data Native and asialo-Tamm-Horsfall glycoproteins Biophysics Oligosaccharides Biochemistry Artocarpus Mucoproteins Structural Biology Lectins Uromodulin Genetics Humans Molecular Biology chemistry.chemical_classification biology Chemistry Cell Biology biology.organism_classification Precipitin Molecular biology Vicia villosa Vicia Binding properties of GalNAc specific lectins Enzyme Carbohydrate Sequence Blood Group Antigens Jacalin biology.protein lipids (amino acids peptides and proteins) Plant Lectins Glycoprotein Protein Binding |
| Zdroj: | FEBS Letters. 429:323-326 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(98)00617-6 |
| Popis: | The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm-Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B4 (VVA-B4), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc alpha-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B4 and native Sd(a+)THGP-DBA through GalNAc beta--> was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B4 are useful reagents to differentiate between Sd(a+) and Sd(a-) THGP. |
| Databáze: | OpenAIRE |
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