Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos
| Autor: | Zhonghua Liu, Hongmei Wang, Ji Guang Zhang, Qing Ran Kong, Yan Jun Huan, Bing Teng Xie, Jiang Zhu, Hongbin He, Jingyu Li, Zhan Feng Wu |
|---|---|
| Předmět: |
Nuclear Transfer Techniques
Swine Cloning Organism Embryonic Development Gene Expression Fertilization in Vitro Biology Decitabine Hydroxamic Acids Epigenesis Genetic Embryo Culture Techniques Gene expression medicine Animals Epigenetics Somatic cell nuclear transfer Pig DNA methylation Reprogramming Methylation Epigenetic modification agents Cellular Reprogramming Molecular biology Histone Deacetylase Inhibitors DNA demethylation Trichostatin A Azacitidine Animal Science and Zoology Original Article medicine.drug |
| Zdroj: | ResearcherID The Journal of Reproduction and Development |
| Popis: | Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|