Prevalence and serovar distribution of asymptomatic cervical Chlamydia trachomatis infections as determined by highly sensitive PCR
| Autor: | J. M. M. Walboomers, A. J. C. Van Den Brule, I. Melgers, C. J. L. M. Meijer, R. Roosendaal, J. Lan, C. Burger, O. P. Bleker |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
DNA Bacterial Microbiology (medical) Serotype Sexually transmitted disease medicine.medical_specialty Adolescent Molecular Sequence Data Prevalence Chlamydia trachomatis Biology medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Asymptomatic Uterine Cervical Diseases Internal medicine medicine Humans Mass Screening Outpatient clinic Serotyping Young adult Genotyping Aged DNA Primers Molecular Epidemiology Base Sequence Age Factors Chlamydia Infections Middle Aged Immunology Female medicine.symptom Research Article |
| Zdroj: | Journal of Clinical Microbiology. 33:3194-3197 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.33.12.3194-3197.1995 |
| Popis: | The prevalence rates and serovar distributions of Chlamydia trachomatis cervical infections were investigated in two different groups of women. Group I consisted of 393 asymptomatic young women (aged 17 to 30 years) who were invited to participate in a C. trachomatis screening program. Group II consisted of 734 randomly selected patients (aged 17 to 68 years) attending an inner-city gynecological outpatient clinic. C. trachomatis was detected in cervical scrapes by PCR specific for endogenous plasmid. These plasmid PCR-positive samples were subsequently subjected to genotyping by C. trachomatis-specific omp1 PCR-based restriction fragment length polymorphism analysis (J. Lan, J. M. M. Walboomers, R. Roosendaal, G. J. van Doornum, D. M. MacLaren, C. J. L. M. Meijer, and A. J. C. van den Brule, J. Clin. Microbiol. 31:1060-1065, 1993). The overall prevalence rates of C. trachomatis found in patients younger than 30 years were 9.2 and 11.8% in groups I and II, respectively. A clear age dependency was seen in group II, with the highest prevalence rate (20%) found in patients younger than 20 years, while the rate declined significantly after 30 years of age (5.9%). In women younger than 30 years, the genotyping results showed that serovars E, I, and D (in decreasing order) were frequent in group I, while serovars F, E, and G (in decreasing order) were predominantly found in group II. The study shows that C. trachomatis infections are highly prevalent in asymptomatic young women. The different serovar distributions found most likely reflect the different compositions of the study groups, but additional analysis of the case histories of individual patients suggests that certain serovars might be associated with symptomatic (i.e., serovar G) or asymptomatic (i.e., serovars D and I) infections. |
| Databáze: | OpenAIRE |
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