Croton cajucara BENTH. Leaf Extract Scavenges the Stable Free Radical DPPH and Protects Against Oxidative Stress Induced by Paraquat
| Autor: | Marilene Porawski, Andrea Janz Moreira, Pilar Sanzchez Collado, Javier González-Gallego, Marcelo Tieppo, Norma Possa Marroni, Mirian Salvador |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Paraquat Antioxidant DPPH medicine.medical_treatment Pharmaceutical Science Saccharomyces cerevisiae Pharmacology medicine.disease_cause Thiobarbituric Acid Reactive Substances Lipid peroxidation chemistry.chemical_compound Picrates TBARS medicine Animals Rats Wistar biology Plant Extracts Superoxide Dismutase Biphenyl Compounds Free Radical Scavengers General Medicine Oxidants Rats Plant Leaves Oxidative Stress Liver Biochemistry chemistry Catalase Luminescent Measurements biology.protein Croton Trolox Oxidative stress |
| Zdroj: | Biological and Pharmaceutical Bulletin. 29:161-165 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.29.161 |
| Popis: | Antioxidant effects of extracts from Croton cajucara BENTH. leaves was investigated in different in vitro and in vivo models. Extracts showed inhibitory radical scavenging activity against the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) (75%, 43% and 25% of the standard trolox at 1, 10 and 100 mg/ml, respectively; IC50 218 mg/ml). Percentage survival of Saccharomyces cerevisiae cells treated with 10 mM paraquat increased by 21% and 55%, when 1 mg/ml and 10 mg/ml concentrations of the extract, respectively, were added. The cytosolic concentration of TBARS increased in animals treated with paraquat (+283%), while values did not significantly differ from the controls in rats additionally receiving the leaf extract. Paraquat administration also induced a significant increase in hydroperoxide-initiated chemiluminiscence (+76%), that was partially prevented by the leaf extract (+31%). Liver SOD activity was a 158% higher in animals receiving paraquat as compared to the controls. This effect was abolished by administration of the leaf extract. Paraquat administration did not significantly modify the activity of GPx or catalase. Croton cajucara extract increased GPx and catalase activities in paraquat treated-animals by 342% and 70%, respectively. Our results confirm that Croton cajucara leaf extract present radical scavenging activity and reduce oxidative stress induced by paraquat, suggesting the beneficial use as a potential source of antioxidant agents of natural origin. |
| Databáze: | OpenAIRE |
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