Reduced serum levels of pro-inflammatory chemokines in fragile X syndrome
| Autor: | Nicolas Glaichenhaus, Olfa Khalfallah, Laetitia Davidovic, Susana Barbosa, Emanuela Martinuzzi, R. Frank Kooy, Patricia Bermudez-Martin, Anke Van Dijck, Cyprien Gilet, Ellen Elinck |
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| Přispěvatelé: | Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty congenital hereditary and neonatal diseases and abnormalities Neurology Adolescent [SDV]Life Sciences [q-bio] Intellectual disability Clustering lcsh:RC346-429 Young Adult 03 medical and health sciences 0302 clinical medicine medicine Humans Neurochemistry Child Pathological lcsh:Neurology. Diseases of the nervous system 030304 developmental biology 0303 health sciences business.industry General Medicine Autism spectrum disorders Prognosis medicine.disease 3. Good health Fragile X syndrome Case-Control Studies Child Preschool Immunology Autism Biomarker (medicine) Cytokines Female Human medicine Neurology (clinical) CCL26 Chemokines business 030217 neurology & neurosurgery Biomarkers Research Article |
| Zdroj: | BMC Neurology, Vol 20, Iss 1, Pp 1-12 (2020) BMC neurology BMC Neurology BMC Neurology, BioMed Central, 2020, 20, ⟨10.1186/s12883-020-01715-2⟩ |
| ISSN: | 1471-2377 |
| DOI: | 10.1186/s12883-020-01715-2 |
| Popis: | Background Fragile X syndrome (FXS) is the most frequent cause of inherited intellectual disability and the most commonly identified monogenic cause of autism. Recent studies have shown that long-term pathological consequences of FXS are not solely confined to the central nervous system (CNS) but rather extend to other physiological dysfunctions in peripheral organs. To gain insights into possible immune dysfunctions in FXS, we profiled a large panel of immune-related biomarkers in the serum of FXS patients and healthy controls. Methods We have used a sensitive and robust Electro Chemi Luminescence (ECL)-based immunoassay to measure the levels of 52 cytokines in the serum of n = 25 FXS patients and n = 29 healthy controls. We then used univariate statistics and multivariate analysis, as well as an advanced unsupervised clustering method, to identify combinations of immune-related biomarkers that could discriminate FXS patients from healthy individuals. Results While the majority of the tested cytokines were present at similar levels in FXS patients and healthy individuals, nine chemokines, CCL2, CCL3, CCL4, CCL11, CCL13, CCL17, CCL22, CCL26 and CXCL10, were present at much lower levels in FXS patients. Using robust regression, we show that six of these biomarkers (CCL2, CCL3, CCL11, CCL22, CCL26 and CXCL10) were negatively associated with FXS diagnosis. Finally, applying the K-sparse unsupervised clustering method to the biomarker dataset allowed for the identification of two subsets of individuals, which essentially matched the FXS and healthy control categories. Conclusions Our data show that FXS patients exhibit reduced serum levels of several chemokines and may therefore exhibit impaired immune responses. The present study also highlights the power of unsupervised clustering methods to identify combinations of biomarkers for diagnosis and prognosis in medicine. |
| Databáze: | OpenAIRE |
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