Specific effects of estrogen on growth factor and major histocompatibility complex class II antigen expression in rat aortic allograft
| Autor: | Satoshi Saito, Marie L. Foegh, Noboru Motomura, Peter W. Ramwell, Hong Lou |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine medicine.medical_specialty Time Factors Arteriosclerosis medicine.drug_class medicine.medical_treatment Basic fibroblast growth factor chemistry.chemical_compound Postoperative Complications Antigen Rats Inbred BN Internal medicine medicine Animals Aorta Abdominal Growth Substances Estradiol business.industry Growth factor Histocompatibility Antigens Class II Immunosuppression medicine.disease Immunohistochemistry Rats Transplantation Endocrinology chemistry Rats Inbred Lew Estrogen Surgery Cardiology and Cardiovascular Medicine business |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 114:803-810 |
| ISSN: | 0022-5223 |
| Popis: | Objective: Transplant arteriosclerosis is the major determinant for long-term survival of cardiac transplants. Estradiol treatment inhibits transplant arteriosclerosis. The objective of this study is to determine, in the absence of immunosuppression, the temporal effect of estradiol treatment on the expression of insulin-like growth factor, platelet-derived growth factor, basic fibroblast growth factor, and major histocompatibility complex class II antigen in rat aortic allografts. Methods: Orthotopic abdominal aortic allograft transplantation was performed in male rats with Brown-Norway rats used as donors and Lewis rats as recipients. The recipients ( n = 50) were treated with estradiol 20 μg/kg per day or placebo by osmotic minipump for 2 days before the operation and until they were put to death on postoperative days 1, 3, 7, 14, or 21. The allografts were harvested and insulin-like growth factor, platelet-derived growth factor, basic fibroblast growth factor, and major histocompatibility complex class II antigen expression were determined by immunohistochemical staining. Myointimal thickening was measured by morphometric analysis. Results: In the placebo-treated group, insulin-like growth factor protein progressively increased in all three layers of the allograft, whereas platelet-derived growth factor protein peaked at day 3 and basic fibroblast growth factor protein increased only moderately. Estradiol treatment inhibited the continuous increase in insulin-like growth factor expression, the peak in platelet-derived growth factor expression at day 3, the moderate basic fibroblast growth factor increase at day 21, and major histocompatibility complex class II antigen expression in all three layers of the allograft at day 21. Intimal thickening of allografts from estradiol-treated recipients was twofold to threefold less than that of the placebo-treated recipients at day 21. Conclusion: The development of transplant arteriosclerosis is associated with an early alloimmune response involving sustained increase in insulin-like growth factor expression. Estradiol treatment of the recipient inhibits transplant arteriosclerosis and suppresses insulin-like growth factor and major histocompatibility complex class II antigen expression but not platelet-derived growth factor or basic fibroblast growth factor in all three layers of the allograft during the early posttransplantation alloimmune rejection phase. (J Thorac Cardiovasc Surg 1997;114:803-10) |
| Databáze: | OpenAIRE |
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