MicroRNA-127-3p acts as a tumor suppressor in epithelial ovarian cancer by regulating the BAG5 gene
| Autor: | Linlin Bi, Siwang Wang, Feng Li, Qian Yang, Lin-Rui Duan, Qing Miao, Jiani Yuan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research endocrine system diseases Cell Apoptosis Carcinoma Ovarian Epithelial Biology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Cell Line Tumor microRNA medicine Humans Genes Tumor Suppressor Neoplasm Invasiveness Neoplasms Glandular and Epithelial 3' Untranslated Regions Adaptor Proteins Signal Transducing Cell Proliferation Ovarian Neoplasms Regulation of gene expression Oncogene Lentivirus Bufalin General Medicine Cell cycle Molecular medicine female genital diseases and pregnancy complications Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Female |
| Zdroj: | Oncology Reports. 36:2563-2570 |
| ISSN: | 1791-2431 1021-335X |
| Popis: | In the present study, the tumor-suppressive role of microRNA-127-3p (miR-127-3p) in epithelial ovarian cancer (EOC) was elucidated. Expression of miR-127-3p was examined by quantitative RT-PCR (qRT-PCR) in 9 EOC cell lines and clinical samples from 13 EOC patients. EOC cell lines, OVCAR-3 and Caov-3, were transduced with a lentivirus to overexpress endogenous miR-127-3p. The tumor-suppressive effects of miR-127-3p on EOC proliferation, bufalin sensitivity, invasion and in vivo growth were investigated through proliferation, bufalin sensitivity wound-closure and in vivo tumorigenicity assays, respectively. In addition, luciferase reporter assay and qRT-PCR were conducted to verify whether the Bcl-2-associated athanogene 5 (BAG5) gene was the downstream target of miR-127-3p in EOC. BAG5 was subsequently upregulated in the OVCAR-3 and Caov-3 cells to examine its functional correlation with miR‑127-3p regulation in EOC. The results revealed that in both EOC cell lines and EOC tumor tissues, miR-127-3p was downregulated. Lentiviral-mediated miR-127-3p overexpression exerted tumor-suppressive effects in OVCAR-3 and Caov-3 cells by reducing in vitro proliferation and invasion, increasing bufalin sensitivity, and inhibiting in vivo tumor growth. miR‑127-3p directly regulated the BAG5 gene in EOC. Subsequent BAG5 upregulation ameliorated the tumor-suppressive effects of miR-127-3p overexpression in EOC. In conclusion, miR-127-3p functions as a tumor suppressor in EOC, and its influence on EOC is directly through regulation of BAG5. |
| Databáze: | OpenAIRE |
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