Dependency of γ-secretase complex activity on the structural integrity of the bilayer
| Autor: | Bing K. Jap, Peter J. Walian, Hua Zhou, Shuxia Zhou |
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| Rok vydání: | 2010 |
| Předmět: |
Detergents
Lipid Bilayers Biophysics Biochemistry Article Cell membrane PEN-2 medicine Humans APH-1 Lipid bilayer Molecular Biology biology Chemistry Bilayer Cell Membrane Cholic Acids Cell Biology medicine.anatomical_structure Solubility Membrane protein Membrane protein complex biology.protein Amyloid Precursor Protein Secretases Amyloid precursor protein secretase HeLa Cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 402:291-296 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2010.10.017 |
| Popis: | γ-secretase is a membrane protein complex associated with the production of Aβ peptides that are pathogenic in Alzheimer’s disease. We have characterized the activity of γ-secretase complexes under a variety of detergent solubilization and reconstitution conditions, and the structural state of proteoliposomes by electron microscopy. We found that γ-secretase activity is highly dependent on the physical state or integrity of the membrane bilayer – partial solubilization may increase activity while complete solubilization will abolish it. The activity of well-solubilized γ-secretase can be restored to near native levels when properly reconstituted into a lipid bilayer environment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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