A next-generation sequencing study on mechanisms by which restraint and social instability stresses of male mice alter offspring anxiety-like behavior
| Autor: | Ming-Jiu Luo, Qiao-Qiao Kong, Shu-Fen Ning, Jing-He Tan, Hong-Jie Yuan, Jia Wang, Xiao-Dan Tian |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Restraint Physical Offspring Science Biology Anxiety Hippocampus DNA sequencing Article Andrology Pathogenesis 03 medical and health sciences Mice 0302 clinical medicine Psychology Animals Epigenetics Protein Interaction Maps Social Behavior Gene Fetus Multidisciplinary Behavior Animal Gene Expression Profiling High-Throughput Nucleotide Sequencing Reproducibility of Results Methylation DNA Methylation Spermatozoa 030104 developmental biology Phenotype Gene Expression Regulation DNA methylation Medicine Female 030217 neurology & neurosurgery Stress Psychological Neuroscience |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Pathophysiological mechanisms for depression/anxiety are largely unknown. Evidence for transgenerational transmission of acquired epigenetic marks remains limited. We bred unstressed (US) female mice with adolescently restraint-stressed (RS), social instability-stressed (SI) or US males to produce RS, SI and control F1 offspring, respectively. Compared to controls, while paternal RS decreased anxiety-like behavior (ALB) in both female and male offspring, paternal SI increased ALB only in female offspring. Next-generation sequencing and bioinformatics using RS and SI female offspring identified 5 candidate anxiety-transmitting (CAT) genes; each showed a consistent pattern of DNA methylation from F0 spermatozoa through F1 blastocysts to fetal and adult hippocampi. Further analyses validated 4 CAT genes, demonstrated that paternal SI caused ALB differences between male and female offspring through modifying the CAT genes, and indicated a strong correlation between inflammation and ALB pathogenesis and an important function for intronic DNA methylation in regulating ALB-related genes. In conclusion, this study identified important CAT genes and suggested the possibility that stresses on males might alter offspring’s ALB by modifying sperm DNA methylation. |
| Databáze: | OpenAIRE |
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