Developmental potential of aneuploid human embryos cultured beyond implantation
| Autor: | Li Sun, Magdalena Zernicka-Goetz, Bailey A. T. Weatherbee, Yiping Zhan, Xin Tao, Richard T. Scott, Emre Seli, Tianren Wang, Gary D. Smith, Marta N. Shahbazi, Antonio Pellicer, Laura M Keller |
|---|---|
| Přispěvatelé: | Shahbazi, Marta N. [0000-0002-1599-5747], Weatherbee, Bailey A. T. [0000-0002-6825-6278], Seli, Emre [0000-0001-7464-8203], Zernicka-Goetz, Magdalena [0000-0002-7004-2471], Apollo - University of Cambridge Repository, Shahbazi, Marta N [0000-0002-1599-5747], Weatherbee, Bailey AT [0000-0002-6825-6278] |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Embryology 631/136/1455 Chromosomes Human Pair 21 General Physics and Astronomy Aneuploidy Trisomy 02 engineering and technology 631/136/2086/1986 13 14 13/1 Monosomy Pregnancy Chromosome Segregation 38/23 38/22 14/19 lcsh:Science 631/80/641/2002 Multidisciplinary Mosaicism Stem Cells article Trisomy 16 021001 nanoscience & nanotechnology Cadherins medicine.anatomical_structure embryonic structures Female Ploidy 0210 nano-technology animal structures Science Embryonic Development 13/106 13/109 Biology General Biochemistry Genetics and Molecular Biology 38 Andrology 03 medical and health sciences 13/100 Antigens CD medicine Cell Adhesion Humans Cell Lineage Embryo Implantation Genetic Testing Trophoblast General Chemistry Cell Cycle Checkpoints Genes erbB-1 medicine.disease Embryo Mammalian 030104 developmental biology lcsh:Q Chromosome 21 Chromosomes Human Pair 16 |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-17764-7 |
| Popis: | Funder: Weston Havens Foundation; doi: https://doi.org/10.13039/100011223 Aneuploidy, the presence of an abnormal number of chromosomes, is a major cause of early pregnancy loss in humans. Yet, the developmental consequences of specific aneuploidies remain unexplored. Here, we determine the extent of post-implantation development of human embryos bearing common aneuploidies using a recently established culture platform. We show that while trisomy 15 and trisomy 21 embryos develop similarly to euploid embryos, monosomy 21 embryos exhibit high rates of developmental arrest, and trisomy 16 embryos display a hypo-proliferation of the trophoblast, the tissue that forms the placenta. Using human trophoblast stem cells, we show that this phenotype can be mechanistically ascribed to increased levels of the cell adhesion protein E-CADHERIN, which lead to premature differentiation and cell cycle arrest. We identify three cases of mosaicism in embryos diagnosed as full aneuploid by pre-implantation genetic testing. Our results present the first detailed analysis of post-implantation development of aneuploid human embryos. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|