4′-Chlorodiazepam Protects Mitochondria in T98G Astrocyte Cell Line from Glucose Deprivation
| Autor: | Eliana Baez, Gina Guio-Vega, Valentina Echeverria, George E. Barreto, Daniel Andres Sandoval-Rueda |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival DNA Fragmentation Mitochondrion Toxicology medicine.disease_cause Statistics Nonparametric 03 medical and health sciences 0302 clinical medicine medicine Translocator protein Humans Viability assay Receptor Cell Line Transformed Hypolipidemic Agents Membrane Potential Mitochondrial Benzodiazepinones Dose-Response Relationship Drug biology General Neuroscience Mitochondria Cell biology Glucose 030104 developmental biology medicine.anatomical_structure Cell culture Apoptosis Astrocytes biology.protein Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress Astrocyte |
| Zdroj: | Neurotoxicity Research. 32:163-171 |
| ISSN: | 1476-3524 1029-8428 |
| DOI: | 10.1007/s12640-017-9733-x |
| Popis: | The translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor (PBR), is considered an important regulator of steroidogenesis and a potential therapeutic target in neurological disorders. Previous evidence suggests that TSPO ligands can protect cells during injury and prevent apoptosis in central nervous system (CNS) cells. However, its actions on astrocytic cells under metabolic injury are not well understood. In this study, we explored whether 4'-chlorodiazepam (Ro5-4864), a TSPO ligand, might protect astrocyte mitochondria under glucose deprivation. Our results showed that 4'-chlorodiazepam preserved cell viability and reduced nuclear fragmentation in glucose-deprived cells. These effects were accompanied by a reduced production of free radicals and maintenance of mitochondrial functions in cells treated with 4'-chlorodiazepam. Finally, our findings suggest that TSPO might be involved in reducing oxidative stress by preserving mitochondrial functions in astrocytic cells exposed to glucose withdrawal. |
| Databáze: | OpenAIRE |
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