Porcine decellularized diaphragm hydrogel: A new option for skeletal muscle malformations

Autor: Piero G. Pavan, Edoardo Maghin, Monica Giomo, Martina Piccoli, Arben Dedja, Eugenia Carraro, Silvia Todros, Daniele Boso, Nicola Elvassore, Paolo De Coppi
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
CROSS-LINKING
Diaphragmatic hernia
Medicine (miscellaneous)
Skeletal muscle
02 engineering and technology
Research & Experimental Medicine
Extracellular matrix
Tissue engineering
Pharmacology & Pharmacy
Biology (General)
Decellularization
Chemistry
Biomaterial
021001 nanoscience & nanotechnology
DIFFERENTIATION
medicine.anatomical_structure
Medicine
Research & Experimental
tissue engineering
Self-healing hydrogels
0210 nano-technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biocompatibility
QH301-705.5
extracellular matrix
SMALL-INTESTINAL SUBMUCOSA
complex mixtures
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
SATELLITE CELLS
EXTRACELLULAR-MATRIX
medicine
skeletal muscle
HERNIA REPAIR
Science & Technology
diaphragmatic hernia
PROSTHETIC PATCHES
IN-VITRO
Biological product
COLLAGEN
Hydrogel
030104 developmental biology
hydrogel
MICROARCHITECTURE
Biomedical engineering
Zdroj: Biomedicines
Volume 9
Issue 7
Biomedicines, Vol 9, Iss 709, p 709 (2021)
Popis: Hydrogels are biomaterials that, thanks to their unique hydrophilic and biomimetic characteristics, are used to support cell growth and attachment and promote tissue regeneration. The use of decellularized extracellular matrix (dECM) from different tissues or organs significantly demonstrated to be far superior to other types of hydrogel since it recapitulates the native tissue’s ECM composition and bioactivity. Different muscle injuries and malformations require the application of patches or fillers to replenish the defect and boost tissue regeneration. Herein, we develop, produce, and characterize a porcine diaphragmatic dECM-derived hydrogel for diaphragmatic applications. We obtain a tissue-specific biomaterial able to mimic the complex structure of skeletal muscle ECM
we characterize hydrogel properties in terms of biomechanical properties, biocompatibility, and adaptability for in vivo applications. Lastly, we demonstrate that dECM-derived hydrogel obtained from porcine diaphragms can represent a useful biological product for diaphragmatic muscle defect repair when used as relevant acellular stand-alone patch.
Databáze: OpenAIRE
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