The expression and regulation of miR-1 in goat skeletal muscle and satellite cell during muscle growth and development
| Autor: | Yinghui Ling, Hao Wu, Zhu Lu, Ya Liu, Zijun Zhang, Qi Zheng, Fugui Fang, Sui Menghua, Mingxing Chu, Yunhai Zhang, LiJuan Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Satellite Cells Skeletal Muscle Cell Bioengineering Histone Deacetylases Muscle hypertrophy 03 medical and health sciences Western blot medicine Animals Muscle Skeletal Gene medicine.diagnostic_test biology Goats 0402 animal and dairy science Skeletal muscle 04 agricultural and veterinary sciences biology.organism_classification 040201 dairy & animal science HDAC4 Cell biology MicroRNAs 030104 developmental biology medicine.anatomical_structure Animal Science and Zoology Satellite (biology) Function (biology) Biotechnology |
| Zdroj: | Animal Biotechnology. 31:455-462 |
| ISSN: | 1532-2378 1049-5398 |
| DOI: | 10.1080/10495398.2019.1622555 |
| Popis: | MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered in model animals. To explore the function and mechanism of miR-1 in goat, we firstly explored the expression profile of miR-1 in goat tissues and cells. Furthermore, the target gene of miR-1 was predicted, and the relationship between miR-1 and one of its target genes, histone deacetylase 4 (HDAC4), was analyzed through double luciferase reporter assay, real-time PCR, and western blot. It was found that the miR-1 is most abundantly expressed in goat heart and skeletal muscle tissue. Meanwhile, the expression of miR-1 showed an increasing tendency from new-born goats to the 7-month-old goats, and then its expression decreases as the goats mature further. In addition, the expression levels of miR-1 decreased in goat skeletal muscle satellite cells with the algebraic increasing of cells. At last, the results showed that HDAC4 is a target gene of miR-1 in goat, and miR-1 can inhibit the post-transcriptional expression of HDAC4, but had no significant influence on the mRNA level of HDAC4. It was hypothesized that miR-1 promotes muscle development by inhibiting the post-transcriptional expression of HDAC4 in goat. |
| Databáze: | OpenAIRE |
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