| Autor: |
Nassim-Assir, B., Corredera, D., Alvarez-Pardo, R., Dieck, S., Ciirdaeva, E., Schuman, E., Alvarez-Castelao, B. |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
J. Vis. Exp. |
| ISSN: |
1940-087X |
| Popis: |
Understanding protein homeostasis in vivo is key to knowing how the cells work in both physiological and disease conditions. The present protocol describes in vivo labeling and subsequent purification of newly synthesized proteins using an engineered mouse line to direct protein labeling to specific cellular populations. It is an inducible line by Cre recombinase expression of L274G-Methionine tRNA synthetase (MetRS*), enabling azidonorleucine (ANL) incorporation to the proteins, which otherwise will not occur. Using the method described here, it is possible to purify cell-type-specific proteomes labeled in vivo and detect subtle changes in protein content due to sample complexity reduction. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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