Toxicological alterations induced by subacute exposure of silver nanoparticles in Wistar rats
Autor: | Subhayu Nayek, Amie K. Lund, Imesha W. De Silva, Roberto Aguilar, Guido F. Verbeck |
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Rok vydání: | 2020 |
Předmět: |
Male
Silver Metal Nanoparticles 010501 environmental sciences Pharmacology Toxicology 01 natural sciences Silver nanoparticle 03 medical and health sciences chemistry.chemical_compound medicine Animals Particle Size Rats Wistar Cytotoxicity Lung 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification 0303 health sciences Reactive oxygen species Inhalation Exposure Inhalation Glutathione Rats medicine.anatomical_structure Toxicity Tests Subacute chemistry Liver Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Micronucleus test Toxicity Microscopy Electron Scanning Bone marrow |
Zdroj: | Journal of applied toxicology : JATREFERENCES. 41(6) |
ISSN: | 1099-1263 |
Popis: | Silver nanoparticles (AgNPs) have become crucial players in the field of medicine and various other industries. AgNPs have a wide array of applications, which includes production of electronic goods, cosmetics, synthesis of dyes, and printing inks, as well as targeted delivery of drugs to specialized cells inside the body. Even though humans readily come in contact with these particles, the organ-specific accumulation and resulting mechanisms of toxicity induced by inhaled AgNPs are still under investigation. The goal of this study was to determine the organ distribution of inhaled AgNPs and investigate the resulting systemic toxicity. To do this, male Wistar rats were exposed by inhalation to AgNPs for 4 hr/day (200 parts per billion/day) for five consecutive days. The nanoparticles were generated using a laser ablation technique using a soft-landing ion mobility (SLIM) instrument. Inductively coupled plasma mass spectrometric (ICP-MS) analysis showed organ-specific accumulation of the nanoparticles, with the highest concentration present in the lungs, followed by the liver and kidneys. Nanoparticle distribution was characterized in the organs using scanning electron microscopy (SEM) and matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) imaging. Bone marrow cytotoxicity assay of the cells from the femur of rats showed micronuclei formation and signs of cellular cytotoxicity. Moreover, rats displayed increased levels of circulating lactate and glutathione disulphide (GSSG), as determined by liquid chromatography-mass spectrometry (LC-MS) analysis. Collectively, our observations suggest that inhaled subacute exposure to AgNP results in accumulation of AgNPs in the lungs, liver, and kidneys, preferentially, as well as mediates induced systemic toxicity. |
Databáze: | OpenAIRE |
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