A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds
| Autor: | Dang Quang Svend Le, Gang Wang, Charlotte Christie Petersen, Nonghua Lv, Ran Kang, Jørgen Kjems, Muwan Chen, Lijin Zou, Qing Ma, Flemming Besenbacher, Lars Bolund, Yuanlin Zeng, Yonglun Luo, Ming Ding, Thomas G. Jensen, Cody Bünger, William T. Pu, Frederik Dagnæs-Hansen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Homeobox protein NANOG
Time Factors Cellular differentiation Induced Pluripotent Stem Cells Karyotype Cell Culture Techniques Article Immunophenotyping Mice chemistry.chemical_compound Calcification Physiologic Osteogenesis Animals Humans Cell Lineage CD90 RNA Messenger Induced pluripotent stem cell Osteoblasts Multidisciplinary Tissue Scaffolds Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Anatomy Fibroblasts Chondrogenesis Cell biology Phenotype Gene Expression Regulation Polycaprolactone Stem cell |
| Zdroj: | Zou, L, Luo, Y, Chen, M, Wang, G, Ding, M, Petersen, C C, Kang, R, Dagnaes-Hansen, F, Zeng, Y, Lv, N, Ma, Q, Le, D Q S, Besenbacher, F, Bolund, L, Jensen, T G, Kjems, J, Pu, W T & Bünger, C 2013, ' A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds ', Scientific Reports, vol. 3, 2243 . https://doi.org/10.1038/srep02243 Scientific Reports |
| DOI: | 10.1038/srep02243 |
| Popis: | We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|