Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability
| Autor: | Aida Gonzalez-Diaz, Anna Carrera-Salinas, Miguel Pinto, Meritxell Cubero, Arie van der Ende, Jeroen D. Langereis, M. Ángeles Domínguez, Carmen Ardanuy, Paula Bajanca-Lavado, Sara Marti |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Infecções Respiratórias
Multidisciplinary Haemophilus Infections Portugal Whole Genome Sequencing Multi-locus Sequence Type lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] Bacils Genomics Serogroup Serotype f Polymorphism Single Nucleotide Haemophilus influenzae Genomic Instability Bacillus (Bacteria) Genetic Diversity Genòmica Spain Humans Serotyping Bacterial Capsules Genome Bacterial Phylogeny Multilocus Sequence Typing Netherlands |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Scientific Reports, 12 Scientific Reports, 12, 1 |
| ISSN: | 2045-2322 |
| Popis: | Comparative Study Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation. Tis study was funded by Instituto de Salud Carlos III (ISCIII) through the Projects from the Fondo de Investigaciones Sanitarias “PI16/00977” to SM, and CIBER de Enfermedades Respiratorias (CIBERES–CB06/06/0037), co-funded by the European Regional Development Fund/European Social Fund (ERDF/ESF, “Investing in your future”), and CERCA Programme/Generalitat de Catalunya for institutional support. Bioinformatic analysis was supported by an Amazon Web Services (AWS) research grant to SM. AC was supported by FPU grant “FPU16/02202” (Formación de Profesorado Universitario, Ministerio de Educación, Spain), and SM was supported by Miguel Servet contract “CP19/00096” (ISCIII). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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