A heterogeneous set of urea-insoluble proteins in dividing PC12 pheochromocytoma cells is passed on to at least the generation of great-granddaughter cells

Autor:	Darin J. Weber, Philip N. McFadden
Rok vydání:	1995
Předmět:	Molecular mass Protein subunit Protein turnover Buffers PC12 Cells Biochemistry Neoplasm Proteins Rats chemistry.chemical_compound Electrophoresis Solubility chemistry Leucine Urea Animals Bioorganic chemistry Doubling time Carbon Radioisotopes Cell Division Half-Life
Zdroj:	Journal of Protein Chemistry. 14:283-289
ISSN:	1573-4943 0277-8033
Popis:	About 5% of the total cellular protein synthesized in exponentially dividing PC12 phenochromocytoma cells remains insoluble after extractions with aqueous buffer, nonionic detergent, and a strong denaturant, 6 M urea. Single- and double-radiolabel pulse-chase labeling experiments with radioactive leucine indicate that for much of the 6 M urea-insoluble protein there is either a lag between its synthesis and deposition in a urea-insoluble compartment and/or the urea-insoluble protein is comparatively stabilized from destruction. Given the doubling time of PC12 cells, much of the long-lived and urea-insoluble protein of PC12 cells is passed on for at least three generations. Electrophoretic analyses show there are many species of long-lived proteins in the 6 M urea-insoluble fraction, displayed as a near continuum of subunit molecular weights.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d7b8f8f15b93d31ef369d5095564d7e https://doi.org/10.1007/bf01886785 Zobrazit plný text záznamu Full text from SpringerLink