The Incorporation of Radioactive Uridine into the Hepatitis B Antigen of a Chimpanzee

Autor:	Jan Desmyter, Wladyslaw Jóźwiak, Irving Millman, J. Mortelmans, Anna O'Connell
Rok vydání:	1975
Předmět:	Male chemistry.chemical_classification HBsAg RNase P virus diseases RNA Biology Molecular biology General Biochemistry Genetics and Molecular Biology Thin-layer chromatography Uridine Hepatitis B Antigens chemistry.chemical_compound chemistry Antigen Nucleic acid Animals RNA Viral Nucleotide
Zdroj:	Experimental Biology and Medicine. 150:121-125
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-150-38986
Popis:	SummaryRadioactive (3H) uridine was incorporated into RNA isolated from the HBsAg of a chimpanzee carrier. HBsAg was purified by precipitation as an immune complex with the IgG fraction of chimpanzee anti-HBs. RNA was extracted from the washed complex with buffered phenol precipitated with alcohol and four nucleotides were identified by thin layer chromatography after alkali degradation.We acknowledge with appreciation the time and assistance of Dr. Manfred E. Bayer for the electron microscopic examination of the purified HBsAg—anti HBs complexes.Addendum. After this work was prepared for publication we became aware of the possibility that radioactive uridine could have been incorporated into uridine diphosphoglucose and not RNA. We have since repeated the procedure for the isolation of nucleic acid described in methods but included two additional steps. (1) The chimpanzee plasma was treated with 25 μg/ml of RNAse (4000 units/mg, Worthington, Freehold, NJ) and incubated for 15 min at 37°. A control contai...
Databáze:	OpenAIRE
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