Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression
| Autor: | L. M. Wailes, Anthony R. Means, G. S. Mcknight, C. R. Guthrie, Xinyu Zhao, R. P. Matthews |
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| Rok vydání: | 1994 |
| Předmět: |
Transcriptional Activation
Molecular Sequence Data Response element In Vitro Techniques Biology CREB Cell Line Phosphoserine Transactivation Ca2+/calmodulin-dependent protein kinase Cyclic AMP Cyclic AMP Response Element-Binding Protein Promoter Regions Genetic Protein kinase A Molecular Biology Cells Cultured Regulation of gene expression Reporter gene Base Sequence Cell Biology Molecular biology Recombinant Proteins Cell Compartmentation Gene Expression Regulation Calcium-Calmodulin-Dependent Protein Kinases biology.protein Calcium Calcium-Calmodulin-Dependent Protein Kinase Type 2 Oligonucleotide Probes Calcium-Calmodulin-Dependent Protein Kinase Type 4 Research Article |
| Zdroj: | Molecular and Cellular Biology. 14:6107-6116 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.14.9.6107 |
| Popis: | Phosphorylation of CREB (cyclic AMP [cAMP]- response element [CRE]-binding protein) by cAMP-dependent protein kinase (PKA) leads to the activation of many promoters containing CREs. In neurons and other cell types, CREB phosphorylation and activation of CRE-containing promoters can occur in response to elevated intracellular Ca2+. In cultured cells that normally lack this Ca2+ responsiveness, we confer Ca(2+)-mediated activation of a CRE-containing promoter by introducing an expression vector for Ca2+/calmodulin-dependent protein kinase type IV (CaMKIV). Activation could also be mediated directly by a constitutively active form of CaMKIV which is Ca2+ independent. The CaMKIV-mediated gene induction requires the activity of CREB/ATF family members but is independent of PKA activity. In contrast, transient expression of either a constitutively active or wild-type Ca2+/calmodulin-dependent protein kinase type II (CaMKII) fails to mediate the transactivation of the same CRE-containing reporter gene. Examination of the subcellular distribution of transiently expressed CaMKIV and CaMKII reveals that only CaMKIV enters the nucleus. Our results demonstrate that CaMKIV, which is expressed in neuronal, reproductive, and lymphoid tissues, may act as a mediator of Ca(2+)-dependent gene induction. |
| Databáze: | OpenAIRE |
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