Localization of Brachyury (T) in embryonic and extraembryonic tissues during mouse gastrulation
| Autor: | Kimberly E. Inman, Karen M. Downs |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Fetal Proteins
Brachyury Mesoderm Notochord Embryonic Development Embryonic Structures Ectoderm Biology Mice Allantois Antibody Specificity Genetics medicine Animals Tissue Distribution Molecular Biology Primitive streak Myocardium Heart Chorion Gastrula Embryo Mammalian Cell biology Gastrulation Viscera medicine.anatomical_structure Epiblast embryonic structures Endoderm T-Box Domain Proteins Developmental Biology |
| Zdroj: | Gene Expression Patterns. 6:783-793 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.modgep.2006.01.010 |
| Popis: | T-box gene family members have important roles during murine embryogenesis, gastrulation, and organogenesis. Although relatively little is known about how T-box genes are regulated, published gene expression studies have revealed dynamic and specific patterns in both embryonic and extraembryonic tissues of the mouse conceptus. Mutant alleles of the T-box gene Brachyury (T) have identified roles in formation of mesoderm and its derivatives, such as somites and the allantois. However, given the cell autonomous nature of T gene activity and conflicting results of gene expression studies, it has been difficult to attribute a primary function to T in normal allantoic development. We report localization of T protein by sectional immunohistochemistry in both embryonic and extraembryonic tissues during mouse gastrulation, emphasizing T localization within the allantois. T was detected in all previously reported sites within the conceptus, including the primitive streak and its derivatives, nascent embryonic mesoderm, the node and notochord, as well as notochord-associated endoderm and posterior neurectoderm. In addition, we have clarified T within the allantois, where it was first detected in the proximal midline of the late allantoic bud (approximately 7.5 days postcoitum, dpc) and persisted within an expanded midline domain until 6-somite pairs (s; approximately 8.5 dpc). Lastly, we have discovered several novel T sites, including the developing heart, visceral endoderm, extraembryonic ectoderm, and its derivative, chorionic ectoderm. Together, these data provide a unified picture of T in the mammalian conceptus, and demonstrate T's presence in unrelated cell types and tissues in highly dynamic spatiotemporal patterns in both embryonic and extraembryonic tissues. |
| Databáze: | OpenAIRE |
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