Regulation of intracellular trafficking and secretion of adiponectin by myosin II
| Autor: | Robert L. Judd, T.D. Braden, Edward E. Morrison, John C. Dennis, Deepa Bedi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Myosin light-chain kinase Biophysics macromolecular substances Biology Heterocyclic Compounds 4 or More Rings Biochemistry Mice Structure-Activity Relationship 03 medical and health sciences symbols.namesake chemistry.chemical_compound 3T3-L1 Cells Adipocyte Myosin Adipocytes Animals Adiponectin secretion Secretion Molecular Biology Cells Cultured Actin Nonmuscle Myosin Type IIB Dose-Response Relationship Drug Adiponectin Nonmuscle Myosin Type IIA Biological Transport Cell Biology Golgi apparatus Bridged Bicyclo Compounds Heterocyclic 030104 developmental biology chemistry symbols Thiazolidines |
| Zdroj: | Biochemical and Biophysical Research Communications. 490:202-208 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2017.06.021 |
| Popis: | Adiponectin is a protein secreted by white adipocytes that plays an important role in insulin action, energy homeostasis and the development of atherosclerosis. The intracellular localization and trafficking of GLUT4 and leptin in adipocytes has been well studied, but little is known regarding the intracellular trafficking of adiponectin. Recent studies have demonstrated that constitutive adiponectin secretion is dependent on PIP2 levels and the integrity of cortical F-actin. Non-muscle myosin II is an actin-based motor that is associated with membrane vesicles and participates in vesicular trafficking in mammalian cells. Therefore, we investigated the role of myosin II in the trafficking and secretion of adiponectin in 3T3-L1 adipocytes. Confocal microscopy revealed that myosin IIA and IIB were dispersed throughout the cytoplasm of the adipocyte. Both myosin isoforms were localized in the Golgi/TGN region as evidenced by colocalization with the cis-Golgi marker, p115 and the trans-Golgi marker, γ-adaptin. Inhibition of myosin II activity by blebbistatin or actin depolymerization by latrunculin B dispersed myosin IIA and IIB towards the periphery while significantly inhibiting adiponectin secretion. Therefore, the constitutive trafficking and secretion of adiponectin in 3T3-L1 adipocytes occurs by an actin-dependent mechanism that involves the actin-based motors, myosin IIA and IIB. |
| Databáze: | OpenAIRE |
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