In vitro fungicidal photodynamic effect of hypericin on Candida species
| Autor: | David Royo-Díez, Mª Jose Revillo, Antonio Rezusta, Santi Nonell, Carlos E. Semino, Mireia Alemany-Ribes, Manuel P. Paz-Cristobal, Patricia López-Chicón, Carmen Aspiroz, Montserrat Agut, Yolanda Gilaberte |
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| Rok vydání: | 2011 |
| Předmět: |
Antifungal Agents
medicine.medical_treatment Photodynamic therapy Microbial Sensitivity Tests In Vitro Techniques Candida parapsilosis Biochemistry Microbiology chemistry.chemical_compound Species Specificity Candida krusei medicine Humans Photosensitizer Physical and Theoretical Chemistry Candida albicans Perylene Cells Cultured Candida Anthracenes Photosensitizing Agents biology General Medicine biology.organism_classification Molecular biology Corpus albicans Hypericin HaCaT chemistry Photochemotherapy |
| Zdroj: | Photochemistry and photobiology. 88(3) |
| ISSN: | 1751-1097 |
| Popis: | Hypericin is a natural photosensitizer considered for the new generation of photodynamic therapy (PDT) drugs. The aim of this study was to evaluate the in vitro fungicidal effect of hypericin PDT on various Candida spp., assessing its photocytotoxicity to keratinocytes (HaCaT) and dermal fibroblasts (hNDF) to determine possible side effects. A 3 log fungicidal effect was observed at 0.5 McFarland for two Candida albicans strains, Candida parapsilosis and Candida krusei with hypericin concentrations of 0.625, 1.25, 2.5 and 40 μm, respectively, at a fluence of 18 J cm(-2) (LED lamp emitting at 602 ± 10 nm). To obtain a 6 log reduction, significantly higher hypericin concentrations and light doses were needed (C. albicans 5 μM, C. parapsilosis 320 μM and C. krusei 320 μM; light dose 37 J cm(-2)). Keratinocytes and fibroblasts can be preserved by keeping the hypericin concentration below 1 μm and the light dose below 37 J cm(-2). C. albicans appears to be suitable for treatment with hypericin PDT without significant damage to cutaneous cells. |
| Databáze: | OpenAIRE |
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