In vitro antileishmanial activity of sustainable anacardic acid and cardol based silver nanoparticles on L. braziliensis
| Autor: | Thayllan Teixeira Bezerra, Mayara Oliveira de Almeida, Nayane Maria de Amorim Lima, Naya Lúcia de Castro Rodrigues, Viviane Gomes Pereira Ribeiro, Maria Jania Teixeira, Luigi Carbone, Giuseppe Mele, Diego Lomonaco, Selma Elaine Mazzetto |
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| Přispěvatelé: | Teixeira Bezerra, Thayllan, Oliveira de Almeida, Mayara, Maria de Amorim Lima, Nayane, Lúcia de Castro Rodrigues, Naya, Gomes Pereira Ribeiro, Viviane, Jania Teixeira, Maria, Carbone, Luigi, Mele, Giuseppe, Lomonaco, Diego, Elaine Mazzetto, Selma |
| Rok vydání: | 2021 |
| Předmět: |
Leishmaniasi
Resorcinol Silver Silver nanoparticle Antiprotozoal Agents Leishmaniasis Cutaneou Pharmaceutical Science Leishmaniasis Cutaneous Metal Nanoparticles Anacardic acid Resorcinols Cardol 24470) [Silver Nitrate (PubChem CID] Anacardic Acids 167551) [Anacardic acid (PubChem CID] Antiprotozoal Agent 64965) [Thiazolyl Blue – MTT (PubChem CID] 23954) [Silver nanoparticles (PubChem CID] Humans 4311764) [Sodium Borohydride (PubChem CID] 76617) [Cardol (PubChem CID] Human |
| Zdroj: | International journal of pharmaceutics. 619 |
| ISSN: | 1873-3476 |
| Popis: | The search for effective and less toxic drugs for the treatment of Cutaneous Leishmaniasis (CL) is desirable due to the emergence of resistant parasites. The present study shows the preparation, characterization and in vitro antileishmanial activity of green-based silver nanoparticles (AgNPs) with Cashew Nutshell Liquid (CNSL, main constituents: anacardic acid (AA) and cardol (CD). The synthesis of silver nanoparticles was achieved by reduction with sodium borohydride in the presence of anacardic acid or cardol under microwave irradiation (400W, 60°C, 5min) resulting in AgAA and AgCD. In vitro assay showed opposite effects for AgAA and AgCD. While AgAA is highly toxic to macrophages (CC50=6.910µgmL-1) and almost non-toxic for L.braziliensis (IC50=86.61µgmL-1), AgCD results very selective toward killing the parasite (CC50=195.0µgmL-1, IC50=11.54µgmL-1). AA's higher polarity and conical shape easily promote cell lysis by increasing cell permeability, while CD has a protective effect: for that reason, AA and AgAA were not further used for tests. CD (EC50=2.906µgmL-1) had higher ability to kill intracellular amastigotes than AgCD (EC50=16.00µgmL-1), however, less intact cells were seen on isolated CD tests. In addition, considering that NO is one of the critical molecular species for the intracellular control of Leishmania, we used Griess colorimetric test to analyze the effect of treatment with AgCD and CD. Overall, the in vitro antileishmanial tests indicate that AgCD should be further explored as a promising non-toxic treatment for CL. |
| Databáze: | OpenAIRE |
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