Label-Free Comparative Proteomic Analysis Combined with Laser-Capture Microdissection Suggests Important Roles of Stress Responses in the Black Layer of Maize Kernels

Autor: Quanquan Chen, Guoying Wang, Riliang Gu, Yaxin Zhang, Junjie Fu, Zhenxiang Xu, Jianhua Wang, Li Li, Xuemei Du, Ran Huang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Proteomics
0106 biological sciences
0301 basic medicine
Laser Capture Microdissection
maize
Zea mays
01 natural sciences
Article
Catalysis
Endosperm
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
Gene Expression Regulation
Plant
Stress
Physiological
Black layer
Physical and Theoretical Chemistry
KEGG
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Microdissection
Plant Proteins
Label free
Laser capture microdissection
Staining and Labeling
Chemistry
Organic Chemistry
Molecular Sequence Annotation
General Medicine
stress response
proteomic analysis
Computer Science Applications
Cell biology
Metabolic pathway
Matrix-assisted laser desorption/ionization
black layer
maldi-tof ms
Gene Ontology
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Seeds
Peptides
Genome
Plant
seed development
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 4, p 1369 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 4
ISSN: 1422-0067
Popis: The black layer (BL) is traditionally used as an indicator for kernel harvesting in maize, as it turns visibly dark when the kernel reaches physiological maturity. However, the molecular roles of BL in kernel development have not been fully elucidated. In this work, microscopy images showed that BL began to appear at a growth stage earlier than 10 days after pollination (DAP), and its color gradually deepened to become dark as the development period progressed. Scanning electron microscopy observations revealed that BL is a tissue structure composed of several layers of cells that are gradually squeezed and compressed during kernel development. Laser-capture microdissection (LCM) was used to sample BL and its neighboring inner tissue, basal endosperm transfer layer (BETL), and outer tissue, inner epidermis (IEP), from 20 DAP of kernels. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling (MALDI-TOF MS profiling) detected 41, 104, and 120 proteins from LCM-sampled BL, BETL, and IEP, respectively. Gene ontology (GO) analysis indicated that the 41 BL proteins were primarily involved in the response to stress and stimuli. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis found that the BL proteins were enriched in several defense pathways, such as the ascorbate and aldarate metabolic pathways. Among the 41 BL proteins, six were BL-specific proteins that were only detected from BL. Annotations of five BL-specific proteins were related to stress responses. During kernel development, transcriptional expression of most BL proteins showed an increase, followed by a decrease, and reached a maximum zero to 20 DAP. These results suggest a role for BL in stress responses for protecting filial tissue against threats from maternal sides, which helps to elucidate the biological functions of BL.
Databáze: OpenAIRE
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