Ellagic Acid Exerts Anti-proliferation Effects via Modulation of Tgf-Β/Smad3 Signaling in MCF-7 Breast Cancer Cells
| Autor: | Xiao-Feng Jiang, Li-Feng Wang, Yi-Chong Wang, Hong-Sheng Chen, Ming Liu, Ming-Han Bai, Tao Zhang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Cancer Research Pyridines Epidemiology Gene Expression Antineoplastic Agents Biology Retinoblastoma Protein Flow cytometry chemistry.chemical_compound Ellagic Acid Transforming Growth Factor beta medicine Humans Pyrroles MTT assay Smad3 Protein Cell Proliferation medicine.diagnostic_test Cell growth Public Health Environmental and Occupational Health Cell Cycle Checkpoints Cell cycle Isoquinolines Molecular biology Oncology chemistry MCF-7 Cancer cell MCF-7 Cells G1 phase Signal Transduction Ellagic acid |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 15:273-276 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2014.15.1.273 |
| Popis: | Ellagic acid has been shown to inhibit tumor cell growth. However, the underlying molecular mechanisms remain elusive. In this study, our aim was to investigate whether ellagic acid inhibits the proliferation of MCF- 7 human breast cancer cells via regulation of the TGF-β/Smad3 signaling pathway. MCF-7 breast cancer cells were transfected with pEGFP-C3 or pEGFP-C3/Smad3 plasmids, and treated with ellagic acid alone or in combination with SIS3, a specific inhibitor of Smad3 phosphorylation. Cell proliferation was assessed by MTT assay and the cell cycle was detected by flow cytometry. Moreover, gene expression was detected by RT-PCR, real-time PCR and Western blot analysis. The MTT assay showed that SIS3 attenuated the inhibitory activity of ellagic acid on the proliferation of MCF-7 cells. Flow cytometry revealed that ellagic acid induced G0/G1 cell cycle arrest which was mitigated by SIS3. Moreover, SIS3 reversed the effects of ellagic acid on the expression of downstream targets of the TGF-β/Smad3 pathway. In conclusion, ellagic acid leads to decreased phosphorylation of RB proteins mainly through modulation of the TGF-β/Smad3 pathway, and thereby inhibits the proliferation of MCF-7 breast cancer cells. |
| Databáze: | OpenAIRE |
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