Establishment from a human chondrosarcoma of a new immortal cell line with high tumorigenicityin vivo, which is able to form proteoglycan-rich cartilage-like nodules and to respond to insulinin vitro
| Autor: | Yoshiro Takano, Akihiro Kinoshita, Koji Tajima, Masaharu Takigawa, Hai‐Ou ‐O Pan |
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| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
Male Cancer Research medicine.medical_specialty Transplantation Heterologous Cell Chondrosarcoma Mice Nude Bone Neoplasms Sulfur Radioisotopes behavioral disciplines and activities Cell Line Extracellular matrix Mice Culture Techniques Internal medicine mental disorders medicine Animals Humans Insulin Aged biology Sulfates Cartilage Molecular biology In vitro Clone Cells Extracellular Matrix Kinetics Microscopy Electron Endocrinology medicine.anatomical_structure Oncology Proteoglycan Cell culture embryonic structures biology.protein Proteoglycans Collagen sense organs Immortalised cell line Neoplasm Transplantation Fetal bovine serum |
| Zdroj: | International Journal of Cancer. 48:717-725 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910480515 |
| Popis: | The human chondrosarcoma cell line (HCS-2/8) established by our group expresses cartilage phenotypes such as production of cartilage-type proteoglycans and collagen type II, but its tumorigenicity is low. To develop an in vitro experimental system for studies of human chondrosarcomas, a new immortal cell line of human chondrosarcoma, named HCS-2/A, was established from the same tumor. HCS-2/A cells proliferated with a doubling time of 3 1/2 days in a medium containing 20% fetal bovine serum (FBS). This growth rate was comparable to that of HCS-2/8 cells. However, HCS-2/A cells proliferated more rapidly than HCS-2/8 cells in the presence of 2-10% FBS. Like HCS-2/8 cells, HCS-2/A cells had a polygonal shape in sparse cultures and became spherical as they reached confluence, after which they formed nodules composed of multilayered cells and a large quantity of extracellular matrix showing strong metachromasia. The nodules formed by HCS-2/A cells were thicker and also larger in diameter than those formed by HCS-2/8 cells. Electron microscopically, the cells in the nodules resembled chondrocytes in vivo, but each cell had an irregular-shaped nucleus which is a characteristics of tumor cells. The cells actively synthesized "cartilage-specific" large proteoglycans and their level of proteoglycan synthesis was comparable to that of HCS-2/8 cells. Insulin, which stimulates proteoglycan and DNA syntheses in cultured chondrocytes, markedly increased proteoglycan synthesis in HCS-2/A cells. On the other hand, the hormone only slightly increased proteoglycan synthesis in HCS-2/8 cells. Insulin also stimulated DNA synthesis in cultured HCS-2/A cells, but not in HCS-2/8 cells. Immunostaining revealed that HCS-2/A cells produced type-II collagen but not type-I collagen. However, the level of collagen synthesis of HCS-2/A cells was lower than that of HCS-2/8 cells. Inoculation of HCS-2/A cells into athymic mice resulted in the formation of chondrosarcomas that grew faster than those arising from HCS-2/8 cells. |
| Databáze: | OpenAIRE |
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