Protein and surface expression of HCN2 and HCN4 subunits in mesocorticolimbic areas after cocaine sensitization
| Autor: | Priscila Sanabria, Cristina María-Ríos, Carlos A. Jiménez-Rivera, Ana del C. Vaquer-Alicea, Rafael Vázquez-Torres, Aynette Ramos-Cardona, Alan Montiel-Ramos, Bermary Santos-Vera |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Potassium Channels media_common.quotation_subject Cell Gene Expression Article Rats Sprague-Dawley Random Allocation 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cocaine Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Limbic System medicine Animals Prefrontal cortex Sensitization media_common Cerebral Cortex Chemistry Addiction Depolarization Cell Biology Hyperpolarization (biology) Rats Ventral tegmental area Protein Subunits 030104 developmental biology medicine.anatomical_structure Neuroscience 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Neurochem Int |
| ISSN: | 0197-0186 |
| Popis: | The I(h) is a mixed depolarizing current present in neurons which, upon activation by hyperpolarization, modulates neuronal excitability in the mesocorticolimbic (MCL) system, an area which regulates emotions such as pleasure, reward, and motivation. Its biophysical properties are determined by HCN protein expression profiles, specifically HCN subunits 1–4. Previously, we reported that cocaine-induced behavioral sensitization increases HCN2 protein expression in all MCL areas with the Ventral Tegmental Area (VTA) showing the most significant increase. Recent evidence suggests that HCN4 also has an important expression in the MCL system. Although there is a significant expression of HCN channels in the MCL system their role in addictive processes is largely unknown. Thus, in this study we aim to compare HCN2 and HCN4 expression profiles and their cellular compartmental distribution in the MCL system, before and after cocaine sensitization. Surface/intracellular (S/I) ratio analysis indicates that VTA HCN2 subunits are mostly expressed in the cell surface in contrast to other areas tested. Our findings demonstrate that after cocaine sensitization, the HCN2 S/I ratio in the VTA was decreased whereas in the Prefrontal Cortex it was increased. In addition, HCN4 total expression in the VTA was decreased after cocaine sensitization, although the S/I ratio was not altered. Together, these results demonstrate differential cocaine effects on HCN2 and HCN4 protein expression profiles and therefore suggest a diverse I(h) modulation of cellular activity during cocaine addictive processes. |
| Databáze: | OpenAIRE |
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