Drug Response of Head and Neck Tumors in Native-State Histoculture
| Autor: | Hal M. Hoffman, Anna Maria Storniolo, K. T. Robbins, Nissi Varki, Robert M. Hoffman |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Oncology
medicine.medical_specialty Cell Survival medicine.drug_class medicine.medical_treatment Antibiotics In vivo Internal medicine Antineoplastic Combined Chemotherapy Protocols Tumor Cells Cultured Drug response Humans Medicine Neoplasm Staging Cisplatin Chemotherapy business.industry Carcinoma Histological Techniques Head and neck tumors General Medicine Surgery Otorhinolaryngology Head and Neck Neoplasms Fluorouracil Carcinoma Squamous Cell Drug Screening Assays Antitumor business Chemosensitivity assay Cell Division medicine.drug |
| Zdroj: | Archives of Otolaryngology - Head and Neck Surgery. 117:83-86 |
| ISSN: | 0886-4470 |
| DOI: | 10.1001/archotol.1991.01870130089022 |
| Popis: | • We describe a chemosensitivity testing of head and neck tumors, in which a native-state histoculture, ie, a three-dimensional culture system that maintains important in vivo properties, including tissue architecture, was used. Fifteen specimens of head and neck tumors were evaluated for sensitivity to the following drugs: cisplatin (DDP) at concentrations of 1.5, 15, and 37.5 μg/mL; fluorouracil at concentrations of 4.0, 40, and 100μg/mL; and combinations of cisplatin and fluorouracil in corresponding doses. Growth and measurement of drug responses were successfully completed in 10 specimens (five others were contaminated, four of them prior to instituting rigorous antibiotic washes). The results indicated cisplatin sensitivity in five of 10 patients; fluorouracil sensitivity in four of 10 patients; and fluorouracil-cisplatin sensitivity in seven of eight patients. Our preliminary results indicate that the native-state histoculture technique is feasible to test chemosensitivity of head and neck tumors. ( Arch Otolaryngol Head Neck Surg . 1991;117:83-86) |
| Databáze: | OpenAIRE |
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