Identification and characterization of miRNAs and their potential targets in flax

Autor: Godfrey Neutelings, Stéphane Fénart, Simon Hawkins, Anca Lucau-Danila
Přispěvatelé: Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), Institut National de la Recherche Agronomique (INRA)-Université de Lille, Sciences et Technologies, Université Lille Nord de France (COMUE), French Nord-Pas de Calais regional project ARCir Plant Teq 4' Identification des determinants moleculaires de la qualite des fibres de lin, Université de Lille, CNRS, Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Rok vydání: 2012
Předmět:
EXPRESSION
0106 biological sciences
Linum
GENES
MICRORNAS
Physiology
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
RT-PCR
Plant Science
Real-Time Polymerase Chain Reaction
Primary transcript
01 natural sciences
03 medical and health sciences
Gene Expression Regulation
Plant
Flax
microRNA
Stem-loop structure
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Tissue Distribution
Gene
Conserved Sequence
Plant Proteins
030304 developmental biology
Expressed Sequence Tags
Genetics
Regulation of gene expression
0303 health sciences
Expressed sequence tag
Base Sequence
biology
Microarray analysis techniques
PLANT DEVELOPMENT
STEM
CELL-WALLS
biology.organism_classification
Gene regulation
Ion homeostasis
RNA
Plant
MicroRNAs
Target mRNAs
ARABIDOPSIS-THALIANA
LINUM-USITATISSIMUM L
Sequence Alignment
Agronomy and Crop Science
LIGNIN
010606 plant biology & botany
Zdroj: Journal of Plant Physiology
Journal of Plant Physiology, Elsevier, 2012, 169 (17), pp.1754-1766. ⟨10.1016/j.jplph.2012.06.011⟩
ISSN: 0176-1617
Popis: International audience; MicroRNAs (miRNAs) are small non-protein coding regulatory RNAs released after the cleavage of a primary transcript. A computational homology search of expressed sequence tags (ESTs) available in public databases allowed the identification of 20 conserved miRNAs belonging to 13 different families in flax (Linum usitatissimum). most of the miRNAs were 21 nucleotides-long and carried a uracil at the 5' end. They originated from precursor transcripts that vary greatly in length. A single precursor containing 2 different stem-loop structures, each one carrying a member of the miR398 family, was identified for the first time in plants. qRT-PCR analyses of 4 selected miRNAs indicated that all were differentially expressed in flax tissues. The 20 miRNAs could potentially regulate 112 different targets including genes involved in cell wall metabolism. Analyses of pri-miRNA and potential gene expression profiles in a publically available microarray data set allowed the identification of a number of highly opposite pri-miRNA/target gene profiles potentially involved in regulating plantacyanin levels, F-box mediated signalling processes, protein metabolism and ion homeostasis, as well as 6 unknown processes. (C) 2012 Elsevier GmbH. All rights reserved.
Databáze: OpenAIRE
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