Auer rod-like inclusions in multiple myeloma
| Autor: | Michael Schatz, Jan Hastka, A. Willer, Georgia Metzgeroth, Ruediger Hehlmann, W. Back, O. Maywald |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty Plasma Cells Biology Plasma cell Azure Stains law.invention law medicine Humans Multiple myeloma Inclusion Bodies Auer rod Endoplasmic reticulum Bone Marrow Examination Hematology General Medicine Middle Aged medicine.disease Immunohistochemistry Immunoglobulin A Microscopy Electron medicine.anatomical_structure Cytoplasm Electron microscope Multiple Myeloma Amyloid (mycology) Immunostaining |
| Zdroj: | Annals of Hematology. 82:57-60 |
| ISSN: | 1432-0584 0939-5555 |
| DOI: | 10.1007/s00277-002-0574-0 |
| Popis: | We report a case of IgA multiple myeloma, in which the plasma cells showed multiple azurophilic, Auer rod-like intracytoplasmic inclusions in May-Grünwald-Giemsa-stained marrow smears. Cytochemical stainings revealed a strong alpha-N-esterase activity of these inclusions, whereas the reactions for peroxidase, Sudan black, chloroacetate esterase, and PAS were negative. Immunostaining verified IgA-kappa inside the plasma cells. The inclusions, however, were negative. Amyloid and lysozyme were also not detectable. Electron microscopy showed Auer rod-like inclusions with a smooth surface in the neighborhood of a well-developed rough endoplasmic reticulum, but with no direct relation to it. The inclusions showed a fine lamellar substructure, and the periodicity of the filamentous striations was about 10 nm, comparable with the substructure of typical Auer rods. Our findings suggest that the azurophilic inclusions in multiple myeloma are Auer rod-related structures, which likewise consist of active lysosomal enzymes. In contrast to the Auer rods in acute myeloblastic leukemia (AML), however, the inclusions in multiple myeloma consist of typical plasma cell enzymes. |
| Databáze: | OpenAIRE |
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