Changes in lipid metabolism convey acid tolerance in Saccharomyces cerevisiae

Autor: Sakda Khoomrung, Zhongpeng Guo, Lisbeth Olsson, Jens Nielsen
Přispěvatelé: Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Department of Biology and Biological Engineering, Industrial Biotechnology, Chalmers University of Technology, Department of Biology and Biological Engineering, Systems and Synthetic Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark [Lyngby] (DTU), Vinnova Grant [2012-02597], Novo Nordisk Foundation, Biovacsafe Grant [115308], Chalmers University of Technology [Gothenburg, Sweden], Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Danmarks Tekniske Universitet = Technical University of Denmark (DTU)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
lcsh:Biotechnology
Saccharomyces cerevisiae
S. cerevisiae
Weak acids
Biotechnologies
Management
Monitoring
Policy and Law

yeast
brewer s
Applied Microbiology and Biotechnology
lcsh:Fuel
weak acids
sustainable
yeast physiology
oxidative stress
03 medical and health sciences
chemistry.chemical_compound
lcsh:TP315-360
Lipid biosynthesis
lcsh:TP248.13-248.65
Cardiolipin
Levulinic acid
saccharomyces cerevisiae
SDG 7 - Affordable and Clean Energy
Yeast physiology
2. Zero hunger
Phosphatidylethanolamine
biology
ergostérol
Renewable Energy
Sustainability and the Environment

S cerevisiae
Research
tolérance
Lipid metabolism
Phosphatidic acid
biology.organism_classification
Sustainable
Oleic acid
030104 developmental biology
General Energy
Biochemistry
chemistry
Oxidative stress
Biotechnology
Zdroj: Guo, Z, Khoomrung, S, Nielsen, J & Olsson, L 2018, ' Changes in lipid metabolism convey acid tolerance in Saccharomyces cerevisiae ', Biotechnology for Biofuels, vol. 11, 297 . https://doi.org/10.1186/s13068-018-1295-5
Biotechnology for Biofuels
Biotechnology for Biofuels, BioMed Central, 2018, 11 (1), 15 p. ⟨10.1186/s13068-018-1295-5⟩
Biotechnology for Biofuels 1 (11), 15 p.. (2018)
Biotechnology for Biofuels, 2018, 11 (1), 15 p. ⟨10.1186/s13068-018-1295-5⟩
Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-15 (2018)
ISSN: 1754-6834
DOI: 10.1186/s13068-018-1295-5
Popis: Background The yeast Saccharomyces cerevisiae plays an essential role in the fermentation of lignocellulosic hydrolysates. Weak organic acids in lignocellulosic hydrolysate can hamper the use of this renewable resource for fuel and chemical production. Plasma-membrane remodeling has recently been found to be involved in acquiring tolerance to organic acids, but the mechanisms responsible remain largely unknown. Therefore, it is essential to understand the underlying mechanisms of acid tolerance of S. cerevisiae for developing robust industrial strains. Results We have performed a comparative analysis of lipids and fatty acids in S. cerevisiae grown in the presence of four different weak acids. The general response of the yeast to acid stress was found to be the accumulation of triacylglycerols and the degradation of steryl esters. In addition, a decrease in phosphatidic acid, phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine, and an increase in phosphatidylinositol were observed. Loss of cardiolipin in the mitochondria membrane may be responsible for the dysfunction of mitochondria and the dramatic decrease in the rate of respiration of S. cerevisiae under acid stress. Interestingly, the accumulation of ergosterol was found to be a protective mechanism of yeast exposed to organic acids, and the ERG1 gene in ergosterol biosynthesis played a key in ergosterol-mediated acid tolerance, as perturbing the expression of this gene caused rapid loss of viability. Interestingly, overexpressing OLE1 resulted in the increased levels of oleic acid (18:1n-9) and an increase in the unsaturation index of fatty acids in the plasma membrane, resulting in higher tolerance to acetic, formic and levulinic acid, while this change was found to be detrimental to cells exposed to lipophilic cinnamic acid. Conclusions Comparison of lipid profiles revealed different remodeling of lipids, FAs and the unsaturation index of the FAs in the cell membrane in response of S. cerevisiae to acetic, formic, levulinic and cinnamic acid, depending on the properties of the acid. In future work, it will be necessary to combine lipidome and transcriptome analysis to gain a better understanding of the underlying regulation network and interactions between central carbon metabolism (e.g., glycolysis, TCA cycle) and lipid biosynthesis. Electronic supplementary material The online version of this article (10.1186/s13068-018-1295-5) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE