Gossypol Inhibition of Acrosin and Proacrosin, and Oocyte Penetration by Human Spermatozoa
| Autor: | Van der Ven Hh, Straus Jw, Zaneveld Lj, Asok K. Bhattacharyya, Donald P. Waller, Kenneth L. Polakoski, Kennedy Wp |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Swine Hamster Motility Semen In Vitro Techniques Biology Andrology chemistry.chemical_compound Human fertilization Cricetinae Endopeptidases medicine Animals Sperm-Ovum Interactions Acrosin Enzyme Precursors Gossypol Cell Biology General Medicine Anatomy Oocyte Spermatozoa medicine.anatomical_structure Reproductive Medicine chemistry Depression Chemical Fertilization Female Enantiomer Sperm Capacitation |
| Zdroj: | Biology of Reproduction. 29:999-1009 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod29.4.999 |
| Popis: | Gosspyol a known antispermatogenic agent was found to effectively inhibit the highly purified boar sperm proacrosin-acrosin proteinase enzyme system by irreversibly preventing the autoproteolytic conversion of proacrosin to acrosin and reversibly inhibiting acrosin activity. The agent appears to prevent the self-catalyzed by not the acrosin catalyzed activation of proacrosin. In additional experiments brief exposure of human semen to concentrations of gossypol which did not visibly alter spermatozoal motility of forward progression was found to irreversibly inhibit the conversion of proacrosin to acrosin although the activity of the nonzymogen acrosin was not decreased and also to prevent the human spermatozoa from penetrating denuded hamster oocytes. Gossypol inhibition of proacrosin conversion to acrosin closely paralleled the decline in oocyte penetration. Racemic (+or-) gossypol was equally as effective as the enantiomer (+) gossypol. The results suggest that the inhibition of proacrosin conversion to acrosin is a mechanism by which gossypol exerts its antifertility effect at nonspermicidal concentrations and that low levels of gossypol should be tested for their contraceptive action when placed vaginally. (authors) |
| Databáze: | OpenAIRE |
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