Concordance of PD-L1 expression and CD8+ TIL intensity between NSCLC and synchronous brain metastases
| Autor: | Irfan Cicin, Şermin Durak, Sebnem Batur, Hale Basak Caglar, Onur Dulger, Perran Fulden Yumuk, Süheyla Uyar Bozkurt, Buge Oz, Ebru Tastekin, Rengin Ahiskali, Emine Bozkurtlar, Asli Cakir, Rashad Rzazade |
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| Přispěvatelé: | Batur, Sebnem, Dulger, Onur, Durak, Sermin, Yumuk, Perran Fulden, Caglar, Hale Basak, Bozkurtlar, Emine, Bozkurt, Suheyla, Tastekin, Ebru, Cicin, Irfan, Ahiskali, Rengin, Rzazade, Rashad, Cakir, Asli, Oz, Buge, İÜC, Cerrahpaşa Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Lung Neoplasms CD8-Positive T-Lymphocytes NSCLC B7-H1 Antigen Neoplasms Multiple Primary 0302 clinical medicine Carcinoma Non-Small-Cell Lung Tumor Microenvironment brain metastasis tumor immunology lcsh:R5-920 biology Brain Neoplasms hemic and immune systems General Medicine Middle Aged medicine.anatomical_structure tumor-infiltrating lymphocytes SAFETY 030220 oncology & carcinogenesis Immunohistochemistry Female lcsh:Medicine (General) Research Article PD-L1 CD3 chemical and pharmacologic phenomena 03 medical and health sciences Carcinoma medicine Humans CD8 lymphocytes Programmed death-ligand 1 TILs Aged Lung Tumor-infiltrating lymphocytes business.industry medicine.disease 030104 developmental biology non-small-cell lung cancer ANTIBODY Tumor Infiltrating Lymphocytes biology.protein Cancer research business CD8 Brain metastasis |
| Zdroj: | Bosnian Journal of Basic Medical Sciences Bosnian Journal of Basic Medical Sciences (2019) |
| ISSN: | 1840-4812 1512-8601 |
| DOI: | 10.17305/bjbms.2019.4474 |
| Popis: | Yumuk, Perran Fulden/0000-0001-8650-299X; BATUR, SEBNEM/0000-0001-6577-8970; durak, sermin/0000-0001-6741-4345; Bozkurtlar, Emine/0000-0002-1034-9236 WOS:000544365100006 PubMed ID: 31999935 Programmed death-ligand 1 (PD-L1) is suggested to be a predictive biomarker in non-small-cell lung carcinoma (NSCLC). However, the differential expression of PD-L1 in primary lung tumor vs. synchronous metastases, especially brain metastasis (BM), remains unclear. This study assessed the concordance of PD-L1 expression on tumor cells and tumor-infiltrating lymphocytes (TILs) and CD8(+) TIL intensity between primary lung tumors and synchronous BMs from 24 NSCLC patients. PD-L1, CD3, and CD8 positivity was determined by immunohistochemistry (IHC). PD-L1 scoring was based on the proportion of tumor cells with membranous expression of PD-L1 and the cutoff values = 50%. CD3 and CD8 positivity in TILs was evaluated semi-quantitatively and the proportion of CD3+/CD8(+)TILs was determined. PD-L1 expression on tumor cells and TILs was evaluated in relation to CD3+/CD8(+)TIL proportions and the intensity of CD8(+)TILs between the paired primary lung and BM tissues. In the primary lung tumors, PD-L1 positivity was observed in 25%, 37.5%, and 37.5% cases for the cutoff values = 50% respectively. PD-L1 expression on tumor cells was strongly correlated between the paired primary lung and BM tissues, in all cutoff groups. However, PD-L1 expression on TILs and the proportion of CD3+/CD8(+)TILs were not strongly correlated in all three groups between the paired primary lung tumors and BMs. The intensity of CD8(+)TILs was concordant in only 54.16% of the paired primary lung tumors and BMs. This study showed a high concordance of PD-L1 expression in neoplastic cells between primary NSCLC and synchronous BMs. |
| Databáze: | OpenAIRE |
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