Entorhinal cortical defects in Tg2576 mice are present as early as 2-4 months of age
| Autor: | Paul M. Mathews, Helen E. Scharfman, Michael J. Schaner, Aine M. Duffy, Alejandra Magagna-Poveda, Keria Bermudez-Hernandez, Jose Morales-Corraliza |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Aging Mice Inbred Strains Mice Transgenic Plaque Amyloid Neuropathology Article White matter Myelin Retrosplenial cortex Alzheimer Disease Internal medicine mental disorders medicine Extracellular Animals Entorhinal Cortex Magnesium Amyloid beta-Peptides biology Chemistry General Neuroscience Entorhinal cortex Antidromic Disease Models Animal medicine.anatomical_structure Endocrinology nervous system biology.protein Female Neurology (clinical) Geriatrics and Gerontology NeuN Neuroscience Developmental Biology |
| Zdroj: | Neurobiology of aging. 36(1) |
| ISSN: | 1558-1497 |
| Popis: | The entorhinal cortex (EC) is one of the first brain areas to display neuropathology in Alzheimer's disease. A mouse model which simulates amyloid-β (Aβ) neuropathology, the Tg2576 mouse, was used to address these early changes. Here, we show EC abnormalities occur in 2- to 4-month-old Tg2576 mice, an age before Aβ deposition and where previous studies suggest that there are few behavioral impairments. First we show, using a sandwich enzyme-linked immunosorbent assay, that soluble human Aβ40 and Aβ42 are detectable in the EC of 2-month-old Tg2576 mice before Aβ deposition. We then demonstrate that 2- to 4-month-old Tg2576 mice are impaired at object placement, an EC-dependent cognitive task. Next, we show that defects in neuronal nuclear antigen expression and myelin uptake occur in the superficial layers of the EC in 2- to 4-month-old Tg2576 mice. In slices from Tg2576 mice that contained the EC, there were repetitive field potentials evoked by a single stimulus to the underlying white matter, and a greater response to reduced extracellular magnesium ([Mg(2+)]o), suggesting increased excitability. However, deep layer neurons in Tg2576 mice had longer latencies to antidromic activation than wild type mice. The results show changes in the EC at early ages and suggest that altered excitability occurs before extensive plaque pathology. |
| Databáze: | OpenAIRE |
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