Solid-phase extraction of vinblastine and vincristine from plasma and urine: variable drug recoveries due to non-reproducible column packings

Autor:	V. Erdélyi-tóth, A. Hulshoff, J.J.M. Holthuis, D.E.M.M. Vendrig
Rok vydání:	1987
Předmět:	Detection limit Reproducibility Chromatography Chemistry Extraction (chemistry) General Chemistry Urine Vinblastine High-performance liquid chromatography Reference electrode Vincristine medicine Humans Solid phase extraction Chromatography High Pressure Liquid medicine.drug
Zdroj:	Journal of Chromatography B: Biomedical Sciences and Applications. 414:91-100
ISSN:	0378-4347
DOI:	10.1016/0378-4347(87)80027-0
Popis:	A sensitive and selective high-performance liquid chromatographic (HPLC) method for the determination of vinblastine and vincristine in plasma and urine is described. The drugs are isolated from 1.0 ml of the biological fluid with a solid-phase extraction column (Bond-Elut Diol®). The HPLC method was combined with electrochemical detection at +850 mV versus an Ag/AgCl reference electrode. The detection limit is 100 pg for vinblastine and 250 pg for vincristine with a signal-to-noise ratio of 3, which permits the determination of these compounds in biological fluids at the nanogram level. Evaluation of the isolation method revealed that the drug recoveries and the reproducibility of the extraction procedure depend on the batch number of the solid-phase extraction column used.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d510f9fce0edefc808904056f76a108 https://doi.org/10.1016/0378-4347(87)80027-0 Zobrazit plný text záznamu