The Structural Biology Center 19ID undulator beamline: facility specifications and protein crystallographic results
| Autor: | Stephan L. Ginell, Edwin M. Westbrook, R. W. Alkire, Michael Molitsky, Martin A. Walsh, Jack Lazarz, Istvan Naday, R. Zhang, Gerd Rosenbaum, Ruslan Sanishvili, N. E. C. Duke, K. Lazarski, Lisa J. Keefe, Gwyndaf Evans, John Gonczy, Larry Rock, Andrzej Joachimiak, Frank J. Rotella |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Diffraction
Optics and Photonics Nuclear and High Energy Physics Photon Protein Conformation Synchrotron radiation X-ray optics Advanced Photon Source Photon energy Crystallography X-Ray Article User-Computer Interface Optics Molecular Biology Instrumentation Radiation business.industry Chemistry Proteins Signal Processing Computer-Assisted Equipment Design Undulator Equipment Failure Analysis Crystallography Beamline Illinois business Synchrotrons |
| Zdroj: | Journal of Synchrotron Radiation. 13:30-45 |
| ISSN: | 0909-0495 |
| Popis: | The 19ID undulator beamline of the Structure Biology Center has been designed and built to take full advantage of the high flux, brilliance and quality of X-ray beams delivered by the Advanced Photon Source. The beamline optics are capable of delivering monochromatic X-rays with photon energies from 3.5 to 20 keV (3.5-0.6 A wavelength) with fluxes up to 8-18 x 10(12) photons s(-1) (depending on photon energy) onto cryogenically cooled crystal samples. The size of the beam (full width at half-maximum) at the sample position can be varied from 2.2 mm x 1.0 mm (horizontal x vertical, unfocused) to 0.083 mm x 0.020 mm in its fully focused configuration. Specimen-to-detector distances of between 100 mm and 1500 mm can be used. The high flexibility, inherent in the design of the optics, coupled with a kappa-geometry goniometer and beamline control software allows optimal strategies to be adopted in protein crystallographic experiments, thus maximizing the chances of their success. A large-area mosaic 3 x 3 CCD detector allows high-quality diffraction data to be measured rapidly to the crystal diffraction limits. The beamline layout and the X-ray optical and endstation components are described in detail, and the results of representative crystallographic experiments are presented. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|