The Effect of Acute and Repeated Stress on CRH-R1 and CRH-R2 mRNA Expression in Pituitaries of Wild Type and CRH Knock-Out Mice
| Autor: | Richard Kvetnansky, Sixtus Hynie, V Klenerová |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Corticotropin-Releasing Hormone Biology CREB Receptors Corticotropin-Releasing Hormone Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Internal medicine polycyclic compounds medicine Transcriptional regulation Animals Cyclic AMP Response Element-Binding Protein Receptor Transcription factor Mice Knockout Regulation of gene expression Wild type Cell Biology General Medicine Mice Inbred C57BL 030104 developmental biology Endocrinology nervous system Pituitary Gland Acute Disease Knockout mouse biology.protein Signal transduction Stress Psychological hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery |
| Zdroj: | Cellular and Molecular Neurobiology. 38:163-169 |
| ISSN: | 1573-6830 0272-4340 |
| DOI: | 10.1007/s10571-017-0556-3 |
| Popis: | The activation of the HPA axis is the endocrine measure of stress responsiveness that is initiated by corticotropin-releasing hormone (CRH). CRH exerts its effects via CRHR1 and CRH-R2 receptors coupled to the cAMP signaling system and this process involves transcription factor cAMP-responsive element-binding protein (CREB).This study investigated the role of CRH and the possible involvement of CREB in gene regulation of CRH receptor, under basal conditions and after stress application in the pituitary. We used wild type (wt +/+) controls and CRH knock-out (CRH-KO -/-) male mice. Using CRH-deficient mice, we were able to investigate the consequences of the lack of the CRH on the expression of CRH receptors and transcriptional regulation mediated by CREB. We estimated the effect of acute (IMO 1×) and repeated (IMO 7×) restraint stressors lasting 30 and 120 min on the expression of mRNA CREB, CRH-R1, and CRH-R2 by qPCR. We found very significant difference in the expression of these peptides under the effect of single and repeated stress in control and CRH-KO mice. Our results indicate that both CRH receptors and CREB might be involved in the regulation of stress response in the pituitary of mice. We propose that regulation of the stress response may be better understood if more were known about the mechanisms of CRH receptor signal transduction and involvement of CREB system. |
| Databáze: | OpenAIRE |
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